Literature DB >> 7262337

Improved separation of motile sperm in asthenospermia and its application to artificial insemination homologous (AIH).

S J Harris, M P Milligan, G M Masson, K J Dennis.   

Abstract

A method is described for the separation of motile sperm from semen by "migration' into a diluent, providing a large area of interface between the semen and the diluent and resulting in a good yield of motile sperm (58%), with a mean percentage of motility of 95%. The method provides a specimen free of seminal plasma and debris and suitable for intrauterine insemination in less than two hours. The motile fraction was used for AIH treatment of 20 couples with a mean of 4 years' infertility, where the female factor had been excluded and the male was asthenospermic. After a mean of six cycles, three pregnancies were achieved. Improvement of the percentage of motility alone did not, in this study, give a significant improvement in conception rates when compared with accepted cycles with the placebo.

Entities:  

Keywords:  Artificial Insemination; Biology; Developed Countries; England; Europe; Examinations And Diagnoses; Genetic Technics; Genitalia; Genitalia, Male; Laboratory Examinations And Diagnoses; Northern Europe; Physiology; Pregnancy; Reproduction; Reproductive Technologies; Semen--analysis; Seminal Vesicles; Sperm Transport; United Kingdom; Urogenital System

Mesh:

Substances:

Year:  1981        PMID: 7262337     DOI: 10.1016/s0015-0282(16)45682-7

Source DB:  PubMed          Journal:  Fertil Steril        ISSN: 0015-0282            Impact factor:   7.329


  7 in total

1.  Influence of swim-up method on the recovery of spermatozoa from different types of semen samples.

Authors:  S K Adiga; P Kumar
Journal:  J Assist Reprod Genet       Date:  2001-03       Impact factor: 3.412

2.  Glass wool-filtered spermatozoa and their oocyte penetrating capacity.

Authors:  N Rana; R S Jeyendran; W J Holmgren; C Rotman; L J Zaneveld
Journal:  J In Vitro Fert Embryo Transf       Date:  1989-10

3.  Development of a microchamber which spontaneously selects high-quality sperm for use in in vitro fertilization or micromanipulation.

Authors:  C H Lih; M Obasaju; C McCaffrey; J W Gordon
Journal:  J Assist Reprod Genet       Date:  1996-09       Impact factor: 3.412

4.  The complement regulatory proteins CD55 (decay accelerating factor) and CD59 are expressed on the inner acrosomal membrane of human spermatozoa as well as CD46 (membrane cofactor protein).

Authors:  J A Cummerson; B F Flanagan; D G Spiller; P M Johnson
Journal:  Immunology       Date:  2006-07       Impact factor: 7.397

5.  Spermatozoa selection by the swim-up procedure and two-layer percoll gradient centrifugation.

Authors:  Y Evliyaoğlu; U Ciftçi; N Bozdemir
Journal:  Int Urol Nephrol       Date:  1996       Impact factor: 2.370

6.  Factors affecting sperm yield during swim-up.

Authors:  K Purvis; I Egdetveit
Journal:  J Assist Reprod Genet       Date:  1993-02       Impact factor: 3.412

Review 7.  Male-factor infertility and in vitro fertilization.

Authors:  C A Yates; D M De Kretser
Journal:  J In Vitro Fert Embryo Transf       Date:  1987-06
  7 in total

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