Literature DB >> 7262274

Purification of a nuclease from human serum.

E J Zöllner, G Seibert, H Slor, R K Zahn.   

Abstract

The purification procedure for a nuclease from human serum is described. It includes ammonium sulfate precipitation, chromatography on DEAE-Sephadex and on Sephacryl-S 200, and preparative electrophoresis. The enzyme purified about 2000-fold, is homogeneous in a sodium dodecyl sulfate electrophoretic system, where it has a mol. wt of 78,000. The pH optimum lies around pH 6.5; it is a sugar-nonspecific endonuclease.

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Year:  1981        PMID： 7262274     DOI： 10.1007/BF01990041

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  14 in total

1.  [A preservation and preparation process for desoxyribonucleic acids and their raw materials].

Authors:  R K ZAHN; E TIESLER; A K KLEINSCHMIDT; D LANG
Journal:  Biochem Z       Date:  1962

2.  Desoxyribonuclease activity of sera of man and some other species.

Authors:  N B KURNICK
Journal:  Arch Biochem Biophys       Date:  1953-03       Impact factor: 4.013

3.  [Serum deoxyribonucleases and DNA-antibodies in lupus erythematodes (author's transl)].

Authors:  E J Zöllner; B Heicke; R K Zahn
Journal:  Z Klin Chem Klin Biochem       Date:  1974-10

4.  Activity of serum ribonuclease after partial hepatectomy and acute stress in the rat.

Authors:  K Tsukada
Journal:  Biochim Biophys Acta       Date:  1969-07-22

5.  Changes in rat serum ribonuclease during phenobarbital treatment.

Authors:  M C Lechner
Journal:  Biochem Pharmacol       Date:  1972-08-15       Impact factor: 5.858

6.  An endonuclease associated with bovine plasma albumin fraction. A purification and some properties of the enzyme.

Authors:  M Anai; H Haraguchi; Y Takagi
Journal:  J Biol Chem       Date:  1972-01-10       Impact factor: 5.157

7.  The relationship of desoxyribonuclease inhibitor levels in human sera to the occurrence of antinuclear antibodies.

Authors:  P G Frost; P J Lachmann
Journal:  Clin Exp Immunol       Date:  1968-06       Impact factor: 4.330

8.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

9.  The use of ethidium bromide-circular DNA complexes for the fluorometric analysis of breakage and joining of DNA.

Authors:  C Paoletti; J B LePecq; I R Lehman
Journal:  J Mol Biol       Date:  1971-01-14       Impact factor: 5.469

10.  The relationship between human serum and human pancreatic DNase I.

Authors:  J D Love; R R Hewitt
Journal:  J Biol Chem       Date:  1979-12-25       Impact factor: 5.157
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