Literature DB >> 7260402

Can whole blood be used instead of separated low density cells for examination of the mitogen response?

C Nissen, R Albrecht, M Corneo, D Bruun, B Speck.   

Abstract

The response of whole blood and separated low density cells to 3 mitogenic lectins [Phytohaemagglutinin (PHA), Pokeweed mitogen (PWM) and Concanavalin (Con-A)] was tested in 133 normal individual of all ages, including cord blood. The results obtained with the two methods correlated poorly (r = 0.16 for PHA; 0.47 for PWM and 0.42 for Con-A). Thus, the two methods measure different things and results are not comparable.

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Year:  1981        PMID： 7260402     DOI： 10.1007/BF00319930

Source DB:  PubMed          Journal:  Blut        ISSN： 0006-5242


  8 in total

1.  An application of the whole blood microculture technique to the detection of lymphocyte transformation.

Authors:  T Yamada; Y Mizoguchi; T Monna; S Yamamoto; S Morisawa
Journal:  Osaka City Med J       Date:  1978

2.  Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g.

Authors:  A Böyum
Journal:  Scand J Clin Lab Invest Suppl       Date:  1968

3.  Lymphocyte transformation using whole blood cultures: an analysis of responses.

Authors:  D W Paty; D Hughes
Journal:  J Immunol Methods       Date:  1972-11       Impact factor: 2.303

4.  [Lymphocyte stimulation in various age groups].

Authors:  D Lavanchy; M Rosenthal
Journal:  Schweiz Med Wochenschr       Date:  1978-04-15

5.  An analysis of phytohemagglutinin lymphoblastogenesis in whole blood.

Authors:  R A Boudet; P A Morse; L M Watts
Journal:  Surg Gynecol Obstet       Date:  1978-04

6.  Microtechnique for quantitative evaluation of in vitro lymphocyte transformation.

Authors:  U Junge; J Hoekstra; L Wolfe; F Deinhardt
Journal:  Clin Exp Immunol       Date:  1970-09       Impact factor: 4.330

7.  A new micromethod for evaluating lymphocyte responses to phytohemagglutinin: quantitative analysis of the function of thymus-dependent cells.

Authors:  B H Park; R A Good
Journal:  Proc Natl Acad Sci U S A       Date:  1972-02       Impact factor: 11.205

8.  Lymphocyte transformation during pregnancy: an analysis using whole-blood culture.

Authors:  T Yamamoto; H Hirata; H Taniguchi; Y Kawai; A Uematsu; Y Sugiyama
Journal:  Obstet Gynecol       Date:  1980-02       Impact factor: 7.661
  8 in total

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