Growth and differentiation patterns of human acute non lymphoid leukemia in diffusion chambers before and after treatment.

Diffusion chambers (DC) were used to culture bone marrow of ANLL patients firstly at the onset, secondly in complete remission and thirdly in the relapse of the disease. At the onset of the disease an increase in cellular concentration mainly due to the granulocytic differentiation was observed in DC in all ANLL patients, in contrast to no growth in ANLL patients in complete remission and in relapse. The DC technique reveals a marked pathological pattern of proliferation in every phase of ANLL, at the presentation, in complete remission and in relapse.