| Literature DB >> 7260058 |
K L Maltman, J Neuhard, R A Warren.
Abstract
In a nonpermissive host, an amber mutant, am 37, of bacteriophage phi W-14 synthesizes deoxyribonucleic acid (DNA) of considerably greater buoyant density than the DNA synthesized by wild-type phage. The am 37 DNA lacks the hypermodified pyrimidine, alpha-putrescinylthymine (putThy). Instead, it contains a new modified base, 5-[(hydroxymethyl)-O-pyrophosphoryl]uracil (hmPPUra). Extracts of cells infected with wild-type phi W-14 convert the hmPPUra in am 37 DNA to putThy when incubated with putrescine.Entities:
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Year: 1981 PMID： 7260058 DOI： 10.1021/bi00515a043
Source DB: PubMed Journal: Biochemistry ISSN： 0006-2960 Impact factor: 3.162