Toxicology investigations with cell culture systems.

This review concerns some of the cell culture systems that are most frequently used in toxicology investigations. In particular, it sets out to evaluate the effectiveness of these cell culture systems in assessing the toxic potential of chemicals. Metabolic studies and general and specific toxicology investigations are highlighted. Specific toxicology investigations relate to the effects of the tests substances on the highly specialized functions typical of the cell systems chosen. The general toxicology investigations include most of the other studies where differentiated or undifferentiated cells have been used to evaluate the effects of the tested substances on common basic biochemical processes essential for life. Lastly, we have attempted to focus attention on the most promising applications of cell cultures in toxicology studies for the near future and to identify those areas where further research is needed. Because of the several excellent reviews that already exist, we have decided not to consider cell cultures utilized in screening potential mutagens and carcinogens. We have also excluded investigations of drug therapeutic effects and action mechanisms of drugs.