Literature DB >> 7253699

High-performance liquid chromatography with electrochemical detection as a highly efficient tool for studying catecholaminergic systems. I. Quantification of noradrenaline, adrenaline and dopamine in cultured adrenal medullary cells.

T H Müller, K Unsicker.   

Abstract

A method for simultaneous determinations of noradrenaline (NA), adrenaline (A) and dopamine (DA) in tissue is described. After extraction from crude deproteinized homogenates by the batch mode aluminium oxide method individual catecholamines (CA) were separated in a high-performance liquid chromatography (HPLC) system by isocratic elution with pure aqueous buffers from an octadecyl column. These eluates were directly monitored with a new amperometric detector device (Metrohm VA E 641) in a wall-jet flow cell. Sensitivity, specificity, precision and performance of this relatively simple procedure are characterized. Different applications of this assay with relevance to neurobiological research are presented.

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Year:  1981        PMID： 7253699     DOI： 10.1016/0165-0270(81)90017-0

Source DB:  PubMed          Journal:  J Neurosci Methods        ISSN： 0165-0270            Impact factor:   2.390


  19 in total

1.  Lack of neurotrophin-4 causes selective structural and chemical deficits in sympathetic ganglia and their preganglionic innervation.

Authors:  A Roosen; A Schober; J Strelau; M Bottner; J Faulhaber; G Bendner; S L McIlwrath; H Seller; H Ehmke; G R Lewin; K Unsicker
Journal:  J Neurosci       Date:  2001-05-01       Impact factor: 6.167

2.  Dumb-bell ganglioneuroma of the spine misinterpreted as progressive idiopathic scoliosis. Case report.

Authors:  B L Bauer; H Bauer; P Griss; A Lütcke; D Maroske; H D Mennel; K Unsicker
Journal:  Arch Orthop Trauma Surg       Date:  1989       Impact factor: 3.067

3.  Dopa-release from mouse neuroblastoma clone N 1 E-115 into the culture medium. A test for tyrosine hydroxylase activity.

Authors:  M Bräutigam; R Dreesen; H Herken
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  1982-08       Impact factor: 3.000

4.  Fate of intraocular chromaffin cell suspensions: role of initial nerve growth factor support.

Authors:  I Strömberg; A Hultgårdh-Nilsson; U Hedin; T Ebendal
Journal:  Cell Tissue Res       Date:  1988       Impact factor: 5.249

5.  Strychnine affects catecholamine secretion from bovine adrenal medulla chromaffin cells.

Authors:  D E Dar; O Zinder
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  1995-07       Impact factor: 3.000

6.  Effects of deltamethrin on catecholamine secretion of bovine chromaffin cells.

Authors:  U Bickmeyer; F Weinsberg; H Wiegand
Journal:  Arch Toxicol       Date:  1994       Impact factor: 5.153

7.  Long-term effects of dexamethasone and nerve growth factor on adrenal medullary cells cultured from young adult rats.

Authors:  A S Tischler; R L Perlman; G Nunnemacher; G M Morse; R A DeLellis; H J Wolfe; B E Sheard
Journal:  Cell Tissue Res       Date:  1982       Impact factor: 5.249

8.  C6 glioma cell-conditioned medium induces neurite outgrowth and survival of rat chromaffin cells in vitro: comparison with the effects of nerve growth factor.

Authors:  K Unsicker; J Vey; H D Hofmann; T H Müller; A J Wilson
Journal:  Proc Natl Acad Sci U S A       Date:  1984-04       Impact factor: 11.205

9.  Survival, morphology, and catecholamine storage of chromaffin cells in serum-free culture: evidence for a survival and differentiation promoting activity in medium conditioned by purified chromaffin cells.

Authors:  K Unsicker; G Stahnke; T H Müller
Journal:  Neurochem Res       Date:  1987-11       Impact factor: 3.996

10.  Effects of inorganic mercury (Hg2+) on calcium channel currents and catecholamine release from bovine chromaffin cells.

Authors:  F Weinsberg; U Bickmeyer; H Wiegand
Journal:  Arch Toxicol       Date:  1995       Impact factor: 5.153
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