Literature DB >> 7252122

A simple DNA-dependent fluorescence enhancement assay for cell number.

C J Adams, B Storrie.   

Abstract

A DNA-dependent fluorescence enhancement assay using the dye Hoechst 33258 was developed to quantitate cell number in crude detergent lysates of cells. The assay, which uses a simple filter fluorometer, is sensitive enough to measure 1 x 10(4) cells without interference from mercaptans, culture media, and protein solutions. They assay can be done with freeze-thawed lysates. Fluorescence intensity values are linearly related to cell number in the range of 1 x 10(4) to 2.5 x 10(5) cells for L-929, BHK, and CHO-Kl cell lines.

Entities:  

Mesh：

Substances：

Year:  1981        PMID： 7252122     DOI： 10.1177/29.2.7252122

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN： 0022-1554            Impact factor:   2.479


  8 in total

1.  A simple method for the DNA content assay of culture maintained pancreatic islets of Langerhans.

Authors:  R Calafiore; A Falorni; M V Ambrosini; G Basta; M L Picchio; P Brunetti
Journal:  J Endocrinol Invest       Date:  1991-09       Impact factor: 4.256

2.  A rapid and sensitive fluorometric screening assay using YO-PRO-1 to quantify tumour cell invasion through Matrigel.

Authors:  A Gohla; K Eckert; H R Maurer
Journal:  Clin Exp Metastasis       Date:  1996-10       Impact factor: 5.150

3.  A fluorometric assay for the measurement of endothelial cell density in vitro.

Authors:  Z Parandoosh; C A Bogowitz; M P Nova
Journal:  In Vitro Cell Dev Biol Anim       Date:  1998 Nov-Dec       Impact factor: 2.416

4.  Alterations of pancreatic amylase secretion in the reserpinized rat model of cystic fibrosis. Effects of cerulein and EGF.

Authors:  J Morisset; F L Bérubé; M Vanier; O Benrezzak
Journal:  Int J Pancreatol       Date:  1994-08

5.  Exocytosis of pinocytic contents by Chinese hamster ovary cells.

Authors:  C J Adams; K M Maurey; B Storrie
Journal:  J Cell Biol       Date:  1982-06       Impact factor: 10.539

6.  Changes of Adipose Tissue Morphology and Composition during Late Pregnancy and Early Lactation in Dairy Cows.

Authors:  Ákos Kenéz; Anna Kulcsár; Franziska Kluge; Idir Benbelkacem; Kathrin Hansen; Lena Locher; Ulrich Meyer; Jürgen Rehage; Sven Dänicke; Korinna Huber
Journal:  PLoS One       Date:  2015-05-15       Impact factor: 3.240

7.  Biological Evaluation and In Silico Study of Benzoic Acid Derivatives from Bjerkandera adusta Targeting Proteostasis Network Modules.

Authors:  Katerina Georgousaki; Nikolaos Tsafantakis; Sentiljana Gumeni; George Lambrinidis; Victor González-Menéndez; Jose R Tormo; Olga Genilloud; Ioannis P Trougakos; Nikolas Fokialakis
Journal:  Molecules       Date:  2020-02-04       Impact factor: 4.411

8.  Comoclathrin, a novel potent skin-whitening agent produced by endophytic Comoclathris strains associated with Andalusia desert plants.

Authors:  Katerina Georgousaki; Victor González-Menéndez; José R Tormo; Nikolaos Tsafantakis; Thomas A Mackenzie; Jesús Martín; Sentiljana Gumeni; Ioannis P Trougakos; Fernando Reyes; Nikolas Fokialakis; Olga Genilloud
Journal:  Sci Rep       Date:  2022-01-31       Impact factor: 4.379
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.