Literature DB >> 7252041

The protein sequenator: a new extraction principle and a metal reaction cup.

A S Inglis, M R Rubira, P M Strike.   

Abstract

A simpler and more efficient extraction procedure is proposed for solvent extraction of the Edman degradation procedure in the protein sequenator. The method is less likely than the conventional one to cause wash-out of peptides or proteins from the reaction cup while the very small solvent volumes required substantially decrease the running costs of the instrument. It has been used successfully (98% repetitive yield) with a metal reaction cup in the sequenator.

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Year:  1981        PMID: 7252041     DOI: 10.1016/0165-022x(81)90068-3

Source DB:  PubMed          Journal:  J Biochem Biophys Methods        ISSN: 0165-022X


  2 in total

1.  The primary structure of component 8c-1, a subunit protein of intermediate filaments in wool keratin. Relationships with proteins from other intermediate filaments.

Authors:  L M Dowling; W G Crewther; A S Inglis
Journal:  Biochem J       Date:  1986-06-15       Impact factor: 3.857

2.  The amino acid sequence of component 7c, a type II intermediate-filament protein from wool.

Authors:  L G Sparrow; C P Robinson; D T McMahon; M R Rubira
Journal:  Biochem J       Date:  1989-08-01       Impact factor: 3.857

  2 in total

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