Literature DB >> 724514

Histone-DNA interactions within chromatin. Isolation of histones from DNA-histone adducts induced in nuclei by UV light.

G R Kunkel, H G Martinson.   

Abstract

We have developed a method by which to isolate histones that have been crosslinked to DNA following irradiation of calf thymus nuclei by UV light. The procedure involves separation of protein-DNA adducts from uncrosslinked protein by Sepharose 4B chromatography under dissociating conditions. Histones which are crosslinked to DNA are released by chemical hydrolysis of the DNA and identified by SDS gel electrophoresis. The results indicate that, of the histones, H1 and H3 become crosslinked to the DNA most readily under our irradiation conditions.

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Year:  1978        PMID： 724514      PMCID： PMC342747          DOI： 10.1093/nar/5.11.4263

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  16 in total

1.  Induction of stable protein-deoxyribonucleic acid adducts in Chinese hamster cell chromatin by ultraviolet light.

Authors:  G F Strniste; S C Rall
Journal:  Biochemistry       Date:  1976-04-20       Impact factor: 3.162

2.  The quantitative distribution of pyrimidine nucleotides in calf thymus deoxyribonucleic acid.

Authors:  K BURTON; G B PETERSEN
Journal:  Biochim Biophys Acta       Date:  1957-12

3.  Histone-histone interactions within chromatin. Crosslinking studies using ultraviolet light.

Authors:  H G Martinson; M D Shetlar; B J McCarthy
Journal:  Biochemistry       Date:  1976-05-04       Impact factor: 3.162

4.  Specificity of photochemical cross-linking in protein-nucleic acid complexes: identification of the interacting residues in RNase- pyrimidine nucleotide complex.

Authors:  A Havron; J Sperling
Journal:  Biochemistry       Date:  1977-12-13       Impact factor: 3.162

5.  Effect of cross-linking on the secondary structure of DNA I. Cross-linking by photodimerization.

Authors:  R Herbeck; T J Yu; W L Peticolas
Journal:  Biochemistry       Date:  1976-06-15       Impact factor: 3.162

Review 6.  Chromatin.

Authors:  G Felsenfeld
Journal:  Nature       Date:  1978-01-12       Impact factor: 49.962

7.  Selective dissociation of histones from calf thymus nucleoprotein.

Authors:  H H Ohlenbusch; B M Olivera; D Tuan; N Davidson
Journal:  J Mol Biol       Date:  1967-04-28       Impact factor: 5.469

8.  The binding of deoxyribonucleic acid and histone in native nucleohistone.

Authors:  J A Bartley; R Chalkley
Journal:  J Biol Chem       Date:  1972-06-10       Impact factor: 5.157

9.  Kinetics of the photochemical addition of [35S] cysteine to polynucleotides and nucleic acids.

Authors:  K C Smith; D H Meun
Journal:  Biochemistry       Date:  1968-03       Impact factor: 3.162

10.  Histone-histone interactions within chromatin. Preliminary characterization of presumptive H2B-H2A and H2B-H4 binding.

Authors:  H G Martinson; B J McCarthy
Journal:  Biochemistry       Date:  1976-09-07       Impact factor: 3.162
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  9 in total

1.  Protein-DNA crosslinking in reconstituted nucleohistone, nuclei and whole cells by picosecond UV laser irradiation.

Authors:  D Angelov; S I Dimitrov; V R Russanova; E Keskinova; I G Pashev
Journal:  Nucleic Acids Res       Date:  1988-05-25       Impact factor: 16.971

Review 2.  Contact-site cross-linking agents.

Authors:  G R Kunkel; M Mehrabian; H G Martinson
Journal:  Mol Cell Biochem       Date:  1981-01-20       Impact factor: 3.396

3.  Identification of proteins interacting with newly replicated DNA in SV40-infected cells by UV-induced DNA-protein cross-linking.

Authors:  K Tsutsui; S Watanabe; M Katagiri; T Oda
Journal:  Nucleic Acids Res       Date:  1983-07-25       Impact factor: 16.971

4.  Predominance of core histones in formation of DNA--protein crosslinks in gamma-irradiated chromatin.

Authors:  L K Mee; S J Adelstein
Journal:  Proc Natl Acad Sci U S A       Date:  1981-04       Impact factor: 11.205

5.  Adenovirus type 2 endopeptidase: an unusual phosphoprotein enzyme matured by autocatalysis.

Authors:  P K Chatterjee; S J Flint
Journal:  Proc Natl Acad Sci U S A       Date:  1987-02       Impact factor: 11.205

6.  Nucleosomes will not form on double-stranded RNa or over poly(dA).poly(dT) tracts in recombinant DNA.

Authors:  G R Kunkel; H G Martinson
Journal:  Nucleic Acids Res       Date:  1981-12-21       Impact factor: 16.971

7.  Comparison of the interactions of the adenovirus type 2 major core protein and its precursor with DNA.

Authors:  P K Chatterjee; U C Yang; S J Flint
Journal:  Nucleic Acids Res       Date:  1986-03-25       Impact factor: 16.971

8.  Adenoviral protein VII packages intracellular viral DNA throughout the early phase of infection.

Authors:  P K Chatterjee; M E Vayda; S J Flint
Journal:  EMBO J       Date:  1986-07       Impact factor: 11.598

9.  Hairpin structure within the 3'UTR of DNA polymerase beta mRNA acts as a post-transcriptional regulatory element and interacts with Hax-1.

Authors:  Elzbieta Sarnowska; Ewa A Grzybowska; Krzysztof Sobczak; Ryszard Konopinski; Anna Wilczynska; Maria Szwarc; Tomasz J Sarnowski; Wlodzimierz J Krzyzosiak; Janusz A Siedlecki
Journal:  Nucleic Acids Res       Date:  2007-08-17       Impact factor: 16.971
  9 in total

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