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Literature DB >> 724506

Transcriptional properties of nucleoli isolated from Tetrahymena.

E Gocke, J C Leer, O F Nielsen, O Westergaard.

Abstract

Nucleoli can be isolated from Tetrahymena in a yield of 30-60%. The isolated nucleoli contain rDNA (at least 90% pure) and have a protein to DNA ratio of 30:1. The endogenous RNA-polymerase activity of the r-chromatin has the following properties: (i) The in vitro transcript has a maximal size identical to the in vivo 35S rRNA precursor, demonstrating correct termination on the gene, (ii) 79% of the in vitro transcript is complementary to cDNA of 17S and 25S rRNA which is close to the theoretical maximum for the 35S rRNA precursor, (iii) the elongation rate of the endogenous RNA-polymerase molecules is 9-12 nucleotides/sec, (iv) an average of 4-16 active RNA polymerases are associated with each rDNA molecule depending upon the preparation.

Mesh：

Substances：

Year: 1978 PMID： 724506 PMCID： PMC342729 DOI： 10.1093/nar/5.11.3993

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

26 in total

1. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.

Authors: K BURTON
Journal: Biochem J Date: 1956-02 Impact factor: 3.857

2. The sv40 transcription complex. II. Non-dissociation of protein from SV40 chromatin during transcription.

Authors: M H Green; T L Brooks
Journal: Nucleic Acids Res Date: 1977-12 Impact factor: 16.971

3. The sv40 transcription complex. I. Effect of viral chromatin proteins on endogenous RNA polymerase activity.

Authors: T L Brooks; M H Green
Journal: Nucleic Acids Res Date: 1977-12 Impact factor: 16.971

4. The mechanism by which heparin stimulates transcription in isolated rat liver nuclei. Polyribonucleotide elongation rates and the number of transcribing RNA polymerase molecules present.

Authors: B E Coupar; C J Chesterton
Journal: Eur J Biochem Date: 1977-10-03

5. Preparation of chromatin containing ribosomal deoxyribonucleic acid from the macronucleus of Tetrahymena pyriformis.

Authors: R W Jones
Journal: Biochem J Date: 1978-07-01 Impact factor: 3.857

6. Introduction of hidden breaks during rRNA maturation and ageing in Tetrahymena pyriformis.

Authors: W A Eckert; W Kaffenberger; G Krohne; W W Franke
Journal: Eur J Biochem Date: 1978-07-03

7. Polols and turnover rates of nuclear ribosomal RNA in Tetrahymena pyriformis.

Authors: V Leick; S B Andersen
Journal: Eur J Biochem Date: 1970-07

8. Isolation of ribosomal gene chromatin.

Authors: T Higashinakagawa; H Wahn; R H Reeder
Journal: Dev Biol Date: 1977-02 Impact factor: 3.582

9. Properties of simian virus 40 transcriptional intermediates isolated from nuclei of permissive cells.

Authors: M Shani; E Birkenmeier; E May; N P Salzman
Journal: J Virol Date: 1977-07 Impact factor: 5.103

10. Fidelity of synthesis of preribosomal RNA in isolated nucleoli and nucleolar chromatin.

Authors: N R Ballal; Y C Choi; R Mouche; H Busche
Journal: Proc Natl Acad Sci U S A Date: 1977-06 Impact factor: 11.205

11 in total

1. Termination of transcription in nucleoli isolated from Tetrahymena.

Authors: J C Leer; D Tiryaki; O Westergaard
Journal: Proc Natl Acad Sci U S A Date: 1979-11 Impact factor: 11.205

2. Localization of transcribed regions on extrachromosomal ribosomal RNA genes of Tetrahymena thermophila by R-loop mapping.

Authors: T R Cech; D C Rio
Journal: Proc Natl Acad Sci U S A Date: 1979-10 Impact factor: 11.205

3. DNase I hypersensitive regions correlate with a site-specific endogenous nuclease activity on the r-chromatin of Tetrahymena.

Authors: B Bonven; O Westergaard
Journal: Nucleic Acids Res Date: 1982-12-11 Impact factor: 16.971

Transcriptional properties of nucleoli isolated from Tetrahymena.

1. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.

2. The sv40 transcription complex. II. Non-dissociation of protein from SV40 chromatin during transcription.

3. The sv40 transcription complex. I. Effect of viral chromatin proteins on endogenous RNA polymerase activity.

4. The mechanism by which heparin stimulates transcription in isolated rat liver nuclei. Polyribonucleotide elongation rates and the number of transcribing RNA polymerase molecules present.

5. Preparation of chromatin containing ribosomal deoxyribonucleic acid from the macronucleus of Tetrahymena pyriformis.

6. Introduction of hidden breaks during rRNA maturation and ageing in Tetrahymena pyriformis.

7. Polols and turnover rates of nuclear ribosomal RNA in Tetrahymena pyriformis.

8. Isolation of ribosomal gene chromatin.

9. Properties of simian virus 40 transcriptional intermediates isolated from nuclei of permissive cells.

10. Fidelity of synthesis of preribosomal RNA in isolated nucleoli and nucleolar chromatin.

1. Termination of transcription in nucleoli isolated from Tetrahymena.

2. Localization of transcribed regions on extrachromosomal ribosomal RNA genes of Tetrahymena thermophila by R-loop mapping.

3. DNase I hypersensitive regions correlate with a site-specific endogenous nuclease activity on the r-chromatin of Tetrahymena.

4. An electron microscopic analysis of transcription of nucleolar chromatin isolated from Tetrahymena pyriformis.

5. DNA synthesis, methylation and degradation during conjugation in Tetrahymena thermophila.

6. Effect of lucanthone (miracil D) on transcription of ribosomal RNA genes from Tetrahymena in vivo and in vitro.

7. Studies on transcription termination and splicing of the rRNA precursor in vivo in the presence of proflavine.

8. Replication of the extrachromosomal ribosomal RNA genes of Tetrahymena thermophilia.

9. A site and strand specific nuclease activity with analogies to topoisomerase I frames the rRNA gene of Tetrahymena.

10. In vitro splicing of the ribosomal RNA precursor in isolated nucleoli from Tetrahymena.