Literature DB >> 7240396

Rapid and sensitive method for quantitation of Legionella pneumophila serogroup 1 antigen from human urine.

J A Mangiafico, K W Hedlund, A R Knott.   

Abstract

A reversed passive hemagglutination test was developed to assay relative concentrations of soluble antigen of Legionnaires disease (Legionella pneumophila serogroup 1) in human urine samples. The test is highly sensitive, being able to detect as little as 0.0002 microgram of total antigen. Preliminary results with this test on serial urine and serum samples from a patient with legionellosis show that measurable amounts of antigen are present in urine during the course of the illness. However, no antigen could be detected in the serum of the patient.

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Year:  1981        PMID： 7240396      PMCID： PMC273900          DOI： 10.1128/jcm.13.5.843-845.1981

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  6 in total

1.  REVERSED PASSIVE HAEMAGGLUTINATION SYSTEMS FOR THE ESTIMATION OF TETANUS TOXINS AND ANTITOXINS.

Authors:  R J COOK
Journal:  Immunology       Date:  1965-01       Impact factor: 7.397

2.  Primary isolation media for Legionnaires disease bacterium.

Authors:  J C Feeley; G W Gorman; R E Weaver; D C Mackel; H W Smith
Journal:  J Clin Microbiol       Date:  1978-09       Impact factor: 5.948

3.  Rapid, sensitive assay for staphylococcal enterotoxin and a comparison of serological methods.

Authors:  S J Silverman; A R Knott; M Howard
Journal:  Appl Microbiol       Date:  1968-07

4.  Detection of Legionella pneumonophila antigen in urine by enzyme-linked immunospecific assay.

Authors:  B P Berdal; C E Farshy; J C Feeley
Journal:  J Clin Microbiol       Date:  1979-05       Impact factor: 5.948

5.  Legionnaires' disease antigen detected by enzyme-linked immunosorbent assay.

Authors:  R C Tilton
Journal:  Ann Intern Med       Date:  1979-04       Impact factor: 25.391

6.  Immunologic protection against the Legionnaires' disease bacterium in the AKR/J mouse.

Authors:  K W Hedlund; V G McGann; D S Copeland; S F Little; R G Allen
Journal:  Ann Intern Med       Date:  1979-04       Impact factor: 25.391
  6 in total
  5 in total

1.  Detection of soluble Legionella pneumophila antigens in serum and urine specimens by enzyme-linked immunosorbent assay with monoclonal and polyclonal antibodies.

Authors:  W F Bibb; P M Arnow; L Thacker; R M McKinney
Journal:  J Clin Microbiol       Date:  1984-09       Impact factor: 5.948

2.  Retrospective evaluation of the Du Pont radioimmunoassay kit for detection of Legionella pneumophila serogroup 1 antigenuria in humans.

Authors:  M E Aguero-Rosenfeld; P H Edelstein
Journal:  J Clin Microbiol       Date:  1988-09       Impact factor: 5.948

3.  Detection of Legionella antigenuria by reverse passive agglutination.

Authors:  P W Tang; D de Savigny; S Toma
Journal:  J Clin Microbiol       Date:  1982-06       Impact factor: 5.948

4.  Broad-spectrum enzyme-linked immunosorbent assay for detection of Legionella soluble antigens.

Authors:  P W Tang; S Toma
Journal:  J Clin Microbiol       Date:  1986-10       Impact factor: 5.948

5.  Use of monoclonal antibodies in an epidemiological marker system: a retrospective study of lung specimens from the 1976 outbreak of Legionnaires disease in Philadelphia by indirect fluorescent-antibody and enzyme-linked immunosorbent assay methods.

Authors:  S L Brown; W F Bibb; R M McKinney
Journal:  J Clin Microbiol       Date:  1985-01       Impact factor: 5.948
  5 in total

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