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Literature DB >> 7239540

Characterization of a new cell line, XL2, obtained from Xenopus laevis and determination of optimal culture conditions.

Abstract

A new amphibian permanent cell line is described. It is called XL2 and was initiated from Stage 35 tadpoles of Xenopus laevis. The cell line has an epithelioid morphology and most cells can be classified into two populations with respective chromosome modal numbers 36 and 74. Contact inhibition is low. Its growth is vigorous in L15 or MEM medium supplemented with fetal bovine serum. The mean doubling time is 39 hr and the saturation density is 700,000 cells/cm2 at 25 degrees C. The absolute plating efficiency is about 70%. Cell line XL2 is unable to grow in L15 medium containing a macromolecular fraction of fetal bovine serum. Growth is restored if the latter medium is supplemented with 10 micrograms/ml of hypoxanthine. Optimal conditions for the dye exclusion test, for harvesting the cells, and for cloning in petri dishes are described.

Entities: CellLine Chemical Gene Species

Mesh：

Substances：

Year: 1981 PMID： 7239540 DOI： 10.1007/bf02618137

Source DB: PubMed Journal: In Vitro ISSN： 0073-5655

10 in total

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Authors: K WOLF; M C QUIMBY; E A PYLE; R P DEXTER
Journal: Science Date: 1960-12-23 Impact factor: 47.728

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Authors: Johannes Holtfreter
Journal: Wilhelm Roux Arch Entwickl Mech Org Date: 1931-06

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Authors: M Pudney; M G Varma; C J Leake
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Authors: L E Sooy; L Mezger-Freed
Journal: Exp Cell Res Date: 1970-06 Impact factor: 3.905

6. Stable haploid cultured cell lines from frog embryos.

Authors: J J Freed; L Mezger-Freed
Journal: Proc Natl Acad Sci U S A Date: 1970-02 Impact factor: 11.205

7. Amphibian cells in culture. I. Nutritional studies.

Authors: G D Chinchar; J H Sinclair
Journal: J Cell Physiol Date: 1978-09 Impact factor: 6.384

8. In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain.

Authors: T R Chen
Journal: Exp Cell Res Date: 1977-02 Impact factor: 3.905

9. Cold-blooded vertebrate cell and tissue culture.

Authors: K Wolf
Journal: Methods Enzymol Date: 1979 Impact factor: 1.600

10. AMPHIBIAN CELL CULTURE: PERMANENT CELL LINE FROM THE BULLFROG (RANA CATESBEIANA).

Authors: K WOLF; M C QUIMBY
Journal: Science Date: 1964-06-26 Impact factor: 47.728

10 in total

8 in total

1. Protein arginine methyltransferase Prmt5-Mep50 methylates histones H2A and H4 and the histone chaperone nucleoplasmin in Xenopus laevis eggs.

Authors: Carola Wilczek; Raghu Chitta; Eileen Woo; Jeffrey Shabanowitz; Brian T Chait; Donald F Hunt; David Shechter
Journal: J Biol Chem Date: 2011-10-18 Impact factor: 5.157

Characterization of a new cell line, XL2, obtained from Xenopus laevis and determination of optimal culture conditions.

1. Preparation of monolayer cell cultures from tissues of some lower vertebrates.

2. Purine and pyrimidine nucleotides in some mutant human lymphoblasts.

3.

4. Establishment of a cell line (XTC-2) from the South African clawed toad, Xenopus laevis.

5. A serum macromolecule-supplemented medium for frog cell lines.

6. Stable haploid cultured cell lines from frog embryos.

7. Amphibian cells in culture. I. Nutritional studies.

8. In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain.

9. Cold-blooded vertebrate cell and tissue culture.

10. AMPHIBIAN CELL CULTURE: PERMANENT CELL LINE FROM THE BULLFROG (RANA CATESBEIANA).

1. Protein arginine methyltransferase Prmt5-Mep50 methylates histones H2A and H4 and the histone chaperone nucleoplasmin in Xenopus laevis eggs.

2. Localization of aurora A and aurora B kinases during interphase: role of the N-terminal domain.

3. Amphibian cell culture: established fibroblastic line from embryos of the discoglossid frog, Bombina orientalis.

4. Unique organization and involvement of GAGA factors in transcriptional regulation of the Xenopus stromelysin-3 gene.

5. Effect of purine supplementation on the growth of salmonid cell lines in different mammalian sera.

6. Characterization of a novel Xenopus tropicalis cell line as a model for in vitro studies.

7. Developing immortal cell lines from Xenopus embryos, four novel cell lines derived from Xenopus tropicalis.

8. Distribution of XCAP-E and XCAP-D2 in the Xenopus oocyte nucleus.