Literature DB >> 7238835

Acetylcholinesterase activity in an Aedes aegypti cell line.

E Cohen.   

Abstract

Considerable acetylcholinesterase (AChE) activity was detected in an Aedes aegypti established cell line. The enzyme is blocked by 10(-6) M eserine sulfate, displays excess substrate inhibition and slowly hydrolyzes butyrylthiocholine. A 2-fold stimulation of AChE activity was shown after 2 days exposure to 3 x 10(-7) M beta-ecdysone. AChE activity found in the fresh medium is the contribution of the fetal calf serum portion. A direct relationship between levels of serum and the AChE activity in the cultured cells was demonstrated.

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Year:  1981        PMID： 7238835     DOI： 10.1007/bf01959903

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  11 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Induction of acetylcholinesterase activity by beta-ecdysone in a Drosophila cell line.

Authors:  P Cherbas; L Cherbas; C M Williams
Journal:  Science       Date:  1977-07-15       Impact factor: 47.728

3.  Ecdysterone and acetylcholinesterase activity in cultured Drosophila cells. Inducible, non-inducible and constitutive clones or lines.

Authors:  M Best-Belpomme; A M Courgeon
Journal:  FEBS Lett       Date:  1977-10-15       Impact factor: 4.124

4.  Ecdysone-induced changes in morphology and protein synthesis in Drosophila cell cultures.

Authors:  E Berger; R Ringler; S Alahiotis; M Frank
Journal:  Dev Biol       Date:  1978-02       Impact factor: 3.582

5.  Metabolic and hormonal studies on two insect cell lines.

Authors:  E Cohen; L I Gilbert
Journal:  J Insect Physiol       Date:  1972-06       Impact factor: 2.354

6.  Cockroach leg regeneration: effects of ecdysterone in vitro.

Authors:  E P Marks; R A Leopold
Journal:  Science       Date:  1970-01-02       Impact factor: 47.728

7.  Induction of puffs in polytene chromosomes of in vitro cultured salivary glands of Drosophila melanogaster by ecdysone and echysone analogues.

Authors:  M Ashburner
Journal:  Nat New Biol       Date:  1971-04-14

8.  Action of insect hormones at the cellular level. II. Differing sensitivity to beta-ecdysone of several lines and clones of Drosophila melanogaster cells.

Authors:  A M Courgeon
Journal:  Exp Cell Res       Date:  1975-09       Impact factor: 3.905

9.  Effects of - and -ecdysone on in vitro diploid cell multiplication in Drosophila melanogaster.

Authors:  A M Courgeon
Journal:  Nat New Biol       Date:  1972-08-23

10.  beta-Galactosidase is induced by hormone in Drosophila melanogaster cell cultures.

Authors:  M Best-Belpomme; A M Courgeon; A Rambach
Journal:  Proc Natl Acad Sci U S A       Date:  1978-12       Impact factor: 11.205
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  3 in total

1.  Increase in activity of acetylcholinesterase by 20-OH-ecdysone in a Chironomus tentans cell line.

Authors:  Margarethe Spindler-Barth; Heinrich Schmidt; Ulrich Drews; Klaus -Dieter Spindler
Journal:  Rouxs Arch Dev Biol       Date:  1988-10

2.  Cultivation of mosquito cell lines in serum-free media and their effects on dengue virus replication.

Authors:  G Kuno
Journal:  In Vitro       Date:  1983-09

3.  Induction and inhibition of an apparent neuronal phenotype in Spodoptera frugiperda insect cells (Sf21) by chemical agents.

Authors:  Lacey J Jenson; Sally L Paulson; Jeffrey R Bloomquist
Journal:  Invert Neurosci       Date:  2012-07-14
  3 in total

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