Literature DB >> 7228969

A simplified plaque assay for influenza viruses in Madin-Darby kidney (MDCK) cells.

J Huprikar, S Rabinowitz.   

Abstract

A variety of influenza A and B viruses plaque in MDCK cell in trypsin is added only at the time of viral adsorption to the monolayer. Therefore, a conventional soft-agar overlay can be employed without addition of proteolytic enzymes. Plaquing efficiency was comparable to that when embryonated eggs were used to determine infectivity. Finally the method is simple and economical.

Mesh:

Year:  1980        PMID: 7228969     DOI: 10.1016/0166-0934(80)90020-8

Source DB:  PubMed          Journal:  J Virol Methods        ISSN: 0166-0934            Impact factor:   2.014


  28 in total

1.  Lung-resident memory CD8 T cells (TRM) are indispensable for optimal cross-protection against pulmonary virus infection.

Authors:  Tao Wu; Yinghong Hu; Young-Tae Lee; Keith R Bouchard; Alexandre Benechet; Kamal Khanna; Linda S Cauley
Journal:  J Leukoc Biol       Date:  2013-09-04       Impact factor: 4.962

2.  Application of real time RT-PCR for the genetic homogeneity and stability tests of the seed candidates for live attenuated influenza vaccine production.

Authors:  Svetlana Shcherbik; Sheila B Sergent; William G Davis; Bo Shu; John Barnes; Irina Kiseleva; Natalie Larionova; Alexander Klimov; Tatiana Bousse
Journal:  J Virol Methods       Date:  2013-09-18       Impact factor: 2.014

3.  Plaque assay of bovine rotavirus.

Authors:  G Butchaiah; E Lund
Journal:  Acta Vet Scand       Date:  1983       Impact factor: 1.695

4.  Cyclic dipeptides from lactic acid bacteria inhibit proliferation of the influenza A virus.

Authors:  Min-Kyu Kwak; Rui Liu; Jun-Oh Kwon; Min-Kyu Kim; Andrew HyoungJin Kim; Sa-Ouk Kang
Journal:  J Microbiol       Date:  2013-12-19       Impact factor: 3.422

5.  Phosphorylation drives an apoptotic protein to activate antiapoptotic genes: paradigm of influenza A matrix 1 protein function.

Authors:  Umesh Chandra Halder; Rahul Bhowmick; Tapasi Roy Mukherjee; Mukti Kant Nayak; Mamta Chawla-Sarkar
Journal:  J Biol Chem       Date:  2013-04-02       Impact factor: 5.157

6.  Potent Intracellular Knock-Down of Influenza A Virus M2 Gene Transcript by DNAzymes Considerably Reduces Viral Replication in Host Cells.

Authors:  Binod Kumar; Roopali Rajput; Dibya Ranjan Pati; Madhu Khanna
Journal:  Mol Biotechnol       Date:  2015-09       Impact factor: 2.695

7.  Human influenza is more effective than avian influenza at antiviral suppression in airway cells.

Authors:  Alan Chen-Yu Hsu; Ian Barr; Philip M Hansbro; Peter A Wark
Journal:  Am J Respir Cell Mol Biol       Date:  2010-08-12       Impact factor: 6.914

8.  Comparison of Streptococcus zooepidemicus and influenza virus pathogenicity in mice by three pulmonary exposure routes.

Authors:  R L Sherwood; P T Thomas; C Y Kawanishi; J D Fenters
Journal:  Appl Environ Microbiol       Date:  1988-07       Impact factor: 4.792

9.  Interferon regulatory factor 4 sustains CD8(+) T cell expansion and effector differentiation.

Authors:  Shuyu Yao; Bruno Fernando Buzo; Duy Pham; Li Jiang; Elizabeth J Taparowsky; Mark H Kaplan; Jie Sun
Journal:  Immunity       Date:  2013-11-07       Impact factor: 31.745

10.  Quantitation of influenza virus using field flow fractionation and multi-angle light scattering for quantifying influenza A particles.

Authors:  Tatiana Bousse; David A Shore; Cynthia S Goldsmith; M Jaber Hossain; Yunho Jang; Charles T Davis; Ruben O Donis; James Stevens
Journal:  J Virol Methods       Date:  2013-07-31       Impact factor: 2.014

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