Literature DB >> 7226240

A transcriptionally enriched chromatin fraction released by a Mg++ -dependent endogenous nuclease from rat liver.

D R Sargan, P H Butterworth.   

Abstract

When rat liver nuclei are subjected to endogenous nuclease digestion in the presence of 5 mM Mg++, they release a chromatin fraction containing DNA of well defined fragment lengths and DNA-dependent RNA polymerases. The DNA is enriched 4.4-fold for sequences complementary to cDNA prepared to cytoplasmic poly-A-containing RNA when compared with whole cell DNA. This method of obtaining a transcriptionally enriched chromatin fraction is compared with other reported nuclease methods.

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Year:  1981        PMID： 7226240     DOI： 10.1016/0309-1651(81)90158-2

Source DB:  PubMed          Journal:  Cell Biol Int Rep        ISSN： 0309-1651


  2 in total

1.  Eukaryotic ternary transcription complexes. I. The release of ternary transcription complexes of RNA polymerases I and II by the endogenous nucleases of rat liver nuclei.

Authors:  D R Sargan; P H Butterworth
Journal:  Nucleic Acids Res       Date:  1982-08-11       Impact factor: 16.971

2.  Eukaryotic ternary transcription complexes. II. An approach to the determination of chromatin conformation at the site of transcription.

Authors:  D R Sargan; P H Butterworth
Journal:  Nucleic Acids Res       Date:  1982-08-11       Impact factor: 16.971
  2 in total

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