Literature DB >> 7225508

On the mechanism of hydrogen-deuterium exchange in bacteriorhodopsin.

A G Doukas, A Pande, T Suzuki, R H Callender, B Honig, M Ottolenghi.   

Abstract

Continuous-flow resonance Raman experiments carried out in bacteriorhodopsin show that the exchange of a deuteron on the Schiff base with a proton takes place in times shorter than 3 ms. Exchange mechanisms based on a base-catalyzed deprotonation followed by reprotonation of the Schiff base are excluded. A mechanism is suggested in which a water molecule interacts directly with the Schiff base deuteron in a concerted exchange mechanism. It appears that in the dark, the binding site is more accessible to neutral water molecules than to charged protons.

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Year:  1981        PMID： 7225508      PMCID： PMC1327428          DOI： 10.1016/S0006-3495(81)84889-8

Source DB:  PubMed          Journal:  Biophys J        ISSN： 0006-3495            Impact factor:   4.033


  9 in total

1.  Improved isolation procedures for the purple membrane of Halobacterium halobium.

Authors:  B M Becher; J Y Cassim
Journal:  Prep Biochem       Date:  1975

Review 2.  Bacteriorhodopsin and the purple membrane of halobacteria.

Authors:  W Stoeckenius; R H Lozier; R A Bogomolni
Journal:  Biochim Biophys Acta       Date:  1979-03-14

Review 3.  Hydrogen exchange.

Authors:  S W Englander; N W Downer; H Teitelbaum
Journal:  Annu Rev Biochem       Date:  1972       Impact factor: 23.643

4.  Tunable laser resonance raman spectroscopy of bacteriorhodopsin.

Authors:  A Lewis; J Spoonhower; R A Bogomolni; R H Lozier; W Stoeckenius
Journal:  Proc Natl Acad Sci U S A       Date:  1974-11       Impact factor: 11.205

5.  Photoisomerization, energy storage, and charge separation: a model for light energy transduction in visual pigments and bacteriorhodopsin.

Authors:  B Honig; T Ebrey; R H Callender; U Dinur; M Ottolenghi
Journal:  Proc Natl Acad Sci U S A       Date:  1979-06       Impact factor: 11.205

6.  Dynamics of pH-induced spectral changes in bacteriorhodopsin.

Authors:  S Druckmann; A Samuni; M Ottolenghi
Journal:  Biophys J       Date:  1979-04       Impact factor: 4.033

7.  Test reactions for a stopped-flow apparatus. Reduction of 2,6-dichlorophenolindophenol and potassium ferricyanide by L-ascorbic acid.

Authors:  B Tonomura; H Nakatani; M Ohnishi; J Yamaguchi-Ito; K Hiromi
Journal:  Anal Biochem       Date:  1978-02       Impact factor: 3.365

8.  Molecular flow resonance Raman effect from retinal and rhodopsin.

Authors:  R H Callender; A Doukas; R Crouch; K Nakanishi
Journal:  Biochemistry       Date:  1976-04-20       Impact factor: 3.162

9.  Visual-pigment spectra: implications of the protonation of the retinal Schiff base.

Authors:  B Honig; A D Greenberg; U Dinur; T G Ebrey
Journal:  Biochemistry       Date:  1976-10-19       Impact factor: 3.162
  9 in total
  4 in total

1.  The reaction of hydroxylamine with bacteriorhodopsin studied with mutants that have altered photocycles: selective reactivity of different photointermediates.

Authors:  S Subramaniam; T Marti; S J Rösselet; K J Rothschild; H G Khorana
Journal:  Proc Natl Acad Sci U S A       Date:  1991-03-15       Impact factor: 11.205

2.  Thermodynamic stability of water molecules in the bacteriorhodopsin proton channel: a molecular dynamics free energy perturbation study.

Authors:  B Roux; M Nina; R Pomès; J C Smith
Journal:  Biophys J       Date:  1996-08       Impact factor: 4.033

3.  Evidence for a bound water molecule next to the retinal Schiff base in bacteriorhodopsin and rhodopsin: a resonance Raman study of the Schiff base hydrogen/deuterium exchange.

Authors:  H Deng; L Huang; R Callender; T Ebrey
Journal:  Biophys J       Date:  1994-04       Impact factor: 4.033

4.  Aspartic acid substitutions affect proton translocation by bacteriorhodopsin.

Authors:  T Mogi; L J Stern; T Marti; B H Chao; H G Khorana
Journal:  Proc Natl Acad Sci U S A       Date:  1988-06       Impact factor: 11.205
  4 in total

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