| Literature DB >> 7225467 |
A Martin, M C Biol, E Arrambide, M Richard, P Louisot.
Abstract
The soluble fucosyl-transferase of the rat small intestinal mucosa was purified by passing through a Sephadex G-100 column, then resolved in two isoenzymes F1 (pHi = 4,47) and F2 (pHi = 4,96) by isoelectric focusing. The use in the first step of DEAE-cellulose instead of Sephadex G-100 leads to another component F3 (pHi = 8,70). Kinetic parameters (KM et Vm) for the three isoenzymes are given. The enzymatic mechanism seems to be a bi-bi random type for the three isoenzymes, and F3 appears as the most active species.Entities:
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Year: 1981 PMID： 7225467 DOI： 10.1016/s0300-9084(81)80198-8
Source DB: PubMed Journal: Biochimie ISSN： 0300-9084 Impact factor: 4.079