Literature DB >> 7225326

Manganese ion dependent adenylate cyclase activity in rat testes: purification and properties.

A R Kornblihtt, M M Flawia, H N Torres.   

Abstract

Testicular, soluble adenylate cyclase has been purified by anion-exchange chromatography, gel filtration, and isoelectric focusing. Upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, peak fractions from the latter purification step showed only one polypeptide band with an apparent molecular weight of about 69 000. The following hydrodynamic and molecular parameters have been established for this enzyme: sedimentation constant, 4.3; Stokes radius, 3.95 nm; partial specific volume, 0.74 mL.g(-1); molecular weight, 74 000; fractional ratio, 1.4.

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Year:  1981        PMID： 7225326     DOI： 10.1021/bi00508a033

Source DB:  PubMed          Journal:  Biochemistry        ISSN： 0006-2960            Impact factor:   3.162


  10 in total

Review 1.  Central role of soluble adenylyl cyclase and cAMP in sperm physiology.

Authors:  Mariano G Buffone; Eva V Wertheimer; Pablo E Visconti; Dario Krapf
Journal:  Biochim Biophys Acta       Date:  2014-07-24

2.  G-protein from Medicago sativa: functional association to photoreceptors.

Authors:  J P Muschietti; H E Martinetto; O A Coso; M D Farber; H N Torres; M M Flawia
Journal:  Biochem J       Date:  1993-04-15       Impact factor: 3.857

3.  Protein kinase activities in Neurospora crassa. Molecular properties.

Authors:  G C Glikin; N D Judewicz; H N Torres
Journal:  Mol Cell Biochem       Date:  1982-07-23       Impact factor: 3.396

4.  Cyclic AMP-dependent protein kinase activity in Trypanosoma cruzi.

Authors:  R M Ulloa; E Mesri; M Esteva; H N Torres; M T Téllez-Iñón
Journal:  Biochem J       Date:  1988-10-01       Impact factor: 3.857

5.  Ca2+ calmodulin-dependent protein kinase activity in the ascomycetes Neurospora crassa.

Authors:  R M Ulloa; H N Torres; C M Ochatt; M T Téllez-Iñón
Journal:  Mol Cell Biochem       Date:  1991-04-10       Impact factor: 3.396

6.  Trypanosoma cruzi adenylate cyclase activity. Purification and characterization.

Authors:  M Torruella; M M Flawiá; C Eisenschlos; L Molina y Vedia; C P Rubinstein; H N Torres
Journal:  Biochem J       Date:  1986-02-15       Impact factor: 3.857

7.  Subcellular localization of the enzymes that dephosphorylate myo-inositol polyphosphates in human platelets.

Authors:  L Molina Y Vedia; R D Nolan; E G Lapetina
Journal:  Biochem J       Date:  1988-11-01       Impact factor: 3.857

8.  Characterization of a Gi-protein from Trypanosoma cruzi epimastigote membranes.

Authors:  O A Coso; A Díaz Añel; H Martinetto; J P Muschietti; M Kazanietz; D Fraidenraich; H N Torres; M M Flawia
Journal:  Biochem J       Date:  1992-10-15       Impact factor: 3.857

9.  Soluble adenylate cyclase activity in Neurospora crassa.

Authors:  J A Reig; A R Kornblihtt; M M Flawiá; H N Torres
Journal:  Biochem J       Date:  1982-10-01       Impact factor: 3.857

10.  Adenylate cyclase activity in a higher plant, alfalfa (Medicago sativa).

Authors:  V C Carricarte; G M Bianchini; J P Muschietti; M T Téllez-Iñón; A Perticari; N Torres; M M Flawiá
Journal:  Biochem J       Date:  1988-02-01       Impact factor: 3.857
  10 in total

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