Literature DB >> 7218122

Lectin used in the purification process of Toxoplasma gondii tachyzoites.

S Hoshino-Shimizu, J R Mineo, M E Camargo.   

Abstract

Toxoplasma gondii tachyzoites were purified from mouse peritoneal exudates using lectins to remove the host cells. Best results were obtained with phytohemagglutinin P at a concentration of 0.01% (W/V), which provided a 99.7% pure Toxoplasma suspension. The process was reproducible and easy to perform. Toxoplasmas so obtained were infective and served as sources of high quality antigens for immunofluorescence, hemagglutination, and complement fixation tests.

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Year:  1980        PMID: 7218122

Source DB:  PubMed          Journal:  J Parasitol        ISSN: 0022-3395            Impact factor:   1.276


  5 in total

1.  Specific binding of neoglycoproteins to Toxoplasma gondii tachyzoites.

Authors:  R Robert; P L de la Jarrige; C Mahaza; J Cottin; A Marot-Leblond; J M Senet
Journal:  Infect Immun       Date:  1991-12       Impact factor: 3.441

Review 2.  Lectins and their application to clinical microbiology.

Authors:  M Slifkin; R J Doyle
Journal:  Clin Microbiol Rev       Date:  1990-07       Impact factor: 26.132

3.  Detection of circulating antigens in immunocompromised patients during reactivation of chronic toxoplasmosis.

Authors:  E Candolfi; F Derouin; T Kien
Journal:  Eur J Clin Microbiol       Date:  1987-02       Impact factor: 3.267

4.  Strain-specific antigens of Toxoplasma gondii.

Authors:  P L Ware; L H Kasper
Journal:  Infect Immun       Date:  1987-03       Impact factor: 3.441

5.  A study of the differential respiratory burst activity elicited by promastigotes and amastigotes of Leishmania donovani in murine resident peritoneal macrophages.

Authors:  J Y Channon; M B Roberts; J M Blackwell
Journal:  Immunology       Date:  1984-10       Impact factor: 7.397

  5 in total

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