Literature DB >> 7217076

Polymerization of hemoglobin in sickle trait erythrocytes and lysates.

C T Noguchi, D A Torchia, A N Schechter.   

Abstract

We report 13C/1H magnetic double resonance measurements of polymer in sickle trait erythrocytes and direct measurements of solubility on lysates of these cells as a function of oxygen saturation. Polymerized hemoglobin is detected by these two methods only at oxygen saturation values below 60% and increases to 0.4 of the total hemoglobin at complete deoxygenation. These results confirm the validity of the NMR approach in determining values of polymer fraction. We have also found that the thermodynamic theory, that successfully describes gelation in sickle erythrocytes, does not predict the experimental results for sickle trait erythrocytes and must be refined.

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Year:  1981        PMID： 7217076

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  15 in total

1.  Framing the research agenda for sickle cell trait: building on the current understanding of clinical events and their potential implications.

Authors:  Jonathan C Goldsmith; Vence L Bonham; Clinton H Joiner; Gregory J Kato; Allan S Noonan; Martin H Steinberg
Journal:  Am J Hematol       Date:  2012-02-03       Impact factor: 10.047

2.  Sparing effect of hemoglobin F and hemoglobin A2 on the polymerization of hemoglobin S at physiologic ligand saturations.

Authors:  W N Poillon; B C Kim; G P Rodgers; C T Noguchi; A N Schechter
Journal:  Proc Natl Acad Sci U S A       Date:  1993-06-01       Impact factor: 11.205

3.  Testing of collegiate athletes for sickle cell trait: what we, as genetic counselors should know.

Authors:  Amy Aloe; Lakshmanan Krishnamurti; Beth Kladny
Journal:  J Genet Couns       Date:  2011-04-19       Impact factor: 2.537

Review 4.  Negative health implications of sickle cell trait in high income countries: from the football field to the laboratory.

Authors:  Nigel S Key; Philippe Connes; Vimal K Derebail
Journal:  Br J Haematol       Date:  2015-03-07       Impact factor: 6.998

5.  Saturation transfer electron paramagnetic resonance detection of sickle hemoglobin aggregation during deoxygenation.

Authors:  P Thiyagarajan; M E Johnson
Journal:  Biophys J       Date:  1983-06       Impact factor: 4.033

6.  Polymerization in erythrocytes containing S and non-S hemoglobins.

Authors:  C T Noguchi
Journal:  Biophys J       Date:  1984-06       Impact factor: 4.033

7.  Intracellular polymerization of sickle hemoglobin. Effects of cell heterogeneity.

Authors:  C T Noguchi; D A Torchia; A N Schechter
Journal:  J Clin Invest       Date:  1983-09       Impact factor: 14.808

8.  Effects of a single sickling event on the mechanical fragility of sickle cell trait erythrocytes.

Authors:  Tennille D Presley; Andreas S Perlegas; Lauren E Bain; Samir K Ballas; James S Nichols; Hernan Sabio; Mark T Gladwin; Gregory J Kato; Daniel B Kim-Shapiro
Journal:  Hemoglobin       Date:  2010       Impact factor: 0.849

9.  Effects of alpha-thalassemia and sickle polymerization tendency on the urine-concentrating defect of individuals with sickle cell trait.

Authors:  A K Gupta; K A Kirchner; R Nicholson; J G Adams; A N Schechter; C T Noguchi; M H Steinberg
Journal:  J Clin Invest       Date:  1991-12       Impact factor: 14.808

10.  Molecular and cellular pathogenesis of hemoglobin SC disease.

Authors:  H F Bunn; C T Noguchi; J Hofrichter; G P Schechter; A N Schechter; W A Eaton
Journal:  Proc Natl Acad Sci U S A       Date:  1982-12       Impact factor: 11.205
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