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Literature DB >> 7213617

Phospholipid vesicle formation and transmembrane protein incorporation using octyl glucoside.

L T Mimms, G Zampighi, Y Nozaki, C Tanford, J A Reynolds.

Abstract

Removal of detergent from mixed micelles of egg yolk phosphatidylcholine and octyl glucoside leads to formation of unilamellar phospholipid vesicles with a diameter of about 230 nm. The same procedure applied to mixed micelles containing the transmembrane protein glycophorin A, in addition to lipid and detergent, produces vesicles of the same size with glycophorin incorporated into the bilayer. The pure lipid vesicles are highly impermeable to both anions and cations, and incorporation of up to 220 molecules of glycophorin per vesicle has little effect on permeability.

Entities: Chemical Gene

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Year: 1981 PMID： 7213617 DOI： 10.1021/bi00507a028

Source DB: PubMed Journal: Biochemistry ISSN： 0006-2960 Impact factor: 3.162

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Cited

118 in total

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4. Effect of lengthening lymphocyte function-associated antigen 3 on adhesion to CD2.

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5. Purification and reconstitution of a collagen-binding heat-shock glycoprotein from L6 myoblasts.

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6. Tissue factor pathway inhibitor in activated prothrombin complex concentrates (aPCC) moderates the effectiveness of therapy in some severe hemophilia A patients with inhibitor.

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Phospholipid vesicle formation and transmembrane protein incorporation using octyl glucoside.

1. From liposomes to supported, planar bilayer structures on hydrophilic and hydrophobic surfaces: an atomic force microscopy study.

2. Microheterogeneity of beta-2 glycoprotein I: implications for binding to anionic phospholipids.

3. Histidines, heart of the hydrogen ion channel from influenza A virus: toward an understanding of conductance and proton selectivity.

4. Effect of lengthening lymphocyte function-associated antigen 3 on adhesion to CD2.

5. Purification and reconstitution of a collagen-binding heat-shock glycoprotein from L6 myoblasts.

6. Tissue factor pathway inhibitor in activated prothrombin complex concentrates (aPCC) moderates the effectiveness of therapy in some severe hemophilia A patients with inhibitor.

7. Membrane structural domains. Resolution limits using diphenylhexatriene fluorescence decay.

8. Cross polarization P-31 nuclear magnetic resonance of phospholipids.

9. Inflammatory roles of P-selectin.

10. Contribution of factor VIII light-chain residues 2007-2016 to an activated protein C-interactive site.