Literature DB >> 7213345

The electrophoresis of transferrins in urea/polyacrylamide gels.

R W Evans, J Williams.   

Abstract

The denaturation of transferrin by urea has been studied by (a) electrophoresis in polyacrylamide gels incorporating a urea gradient, (b) measurements of the loss of iron-binding capacity and (c) u.v. difference spectrometry. In human serum transferrin and hen ovotransferrin the N-terminal and C-terminal domains of the iron-free protein were found to denature at different urea concentrations.

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Year:  1980        PMID： 7213345      PMCID： PMC1162034          DOI： 10.1042/bj1890541

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  14 in total

1.  Studies of the binding of different iron donors to human serum transferrin and isolation of iron-binding fragments from the N- and C-terminal regions of the protein.

Authors:  R W Evans; J Williams
Journal:  Biochem J       Date:  1978-08-01       Impact factor: 3.857

2.  Structure and biosynthesis of the ribosomal ribonucleic acids from the oncogenic bacterium Agrobacterium tumefaciens.

Authors:  J M Grienenberger; D Simon
Journal:  Biochem J       Date:  1975-07       Impact factor: 3.857

3.  The detection of four molecular forms of human transferrin during the iron binding process.

Authors:  D G Makey; U S Seal
Journal:  Biochim Biophys Acta       Date:  1976-11-26

4.  Stoichiometric and site characteristics of the binding of iron to human transferrin.

Authors:  P Aisen; A Leibman; J Zweier
Journal:  J Biol Chem       Date:  1978-03-25       Impact factor: 5.157

5.  Conformations of denatured and renatured ovotransferrin.

Authors:  Y Yeh; S Iwai; R E Feeney
Journal:  Biochemistry       Date:  1979-03-06       Impact factor: 3.162

6.  Distribution of iron between the binding sites of transferrin in serum: methods and results in normal human subjects.

Authors:  A Leibman; P Aisen
Journal:  Blood       Date:  1979-06       Impact factor: 22.113

7.  Calorimetric studies on the binding of iron and aluminium to the amino- and carboxyl-terminal fragments of hen ovotransferrin.

Authors:  R W Evans; J W Donovan; J Williams
Journal:  FEBS Lett       Date:  1977-11-01       Impact factor: 4.124

8.  Studies on the binding of iron to transferrin and conalbumin.

Authors:  P Aisen; A Leibman; H A Reich
Journal:  J Biol Chem       Date:  1966-04-25       Impact factor: 5.157

9.  A comparsion of glycopeptides from the transferrins of several species.

Authors:  I Graham; J Williams
Journal:  Biochem J       Date:  1975-02       Impact factor: 3.857

10.  Iron binding to conalbumin. Calorimetric evidence for two distinct species with one bound iron atom.

Authors:  J W Donovan; K D Ross
Journal:  J Biol Chem       Date:  1975-08-10       Impact factor: 5.157
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  20 in total

Review 1.  Bacterial transferrin receptors--structure, function and contribution to virulence.

Authors:  P Williams; E Griffiths
Journal:  Med Microbiol Immunol       Date:  1992       Impact factor: 3.402

2.  Delayed hepcidin response explains the lag period in iron absorption following a stimulus to increase erythropoiesis.

Authors:  D M Frazer; H R Inglis; S J Wilkins; K N Millard; T M Steele; G D McLaren; A T McKie; C D Vulpe; G J Anderson
Journal:  Gut       Date:  2004-10       Impact factor: 23.059

3.  Expression, purification, and characterization of recombinant human transferrin from rice (Oryza sativa L.).

Authors:  Deshui Zhang; Somen Nandi; Paula Bryan; Steve Pettit; Diane Nguyen; Mary Ann Santos; Ning Huang
Journal:  Protein Expr Purif       Date:  2010-05-04       Impact factor: 1.650

4.  The active site is the least stable structure in the unfolding pathway of a multidomain cold-adapted alpha-amylase.

Authors:  Khawar S Siddiqui; Georges Feller; Salvino D'Amico; Charles Gerday; Laura Giaquinto; Ricardo Cavicchioli
Journal:  J Bacteriol       Date:  2005-09       Impact factor: 3.490

5.  The dimerization of half-molecule fragments of transferrin.

Authors:  J Williams; K Moreton
Journal:  Biochem J       Date:  1988-05-01       Impact factor: 3.857

6.  A variant of human transferrin with abnormal properties.

Authors:  R W Evans; J Williams; K Moreton
Journal:  Biochem J       Date:  1982-01-01       Impact factor: 3.857

7.  The influence of pH on the equilibrium distribution of iron between the metal-binding sites of human transferrin.

Authors:  N D Chasteen; J Williams
Journal:  Biochem J       Date:  1981-03-01       Impact factor: 3.857

8.  Meningococcal transferrin-binding proteins A and B show cooperation in their binding kinetics for human transferrin.

Authors:  Russell H Stokes; Jonathan S Oakhill; Christopher L Joannou; Andrew R Gorringe; Robert W Evans
Journal:  Infect Immun       Date:  2005-02       Impact factor: 3.441

9.  Structural analysis of seminal and serum human transferrin by second derivative spectrometry and fluorescence measurements.

Authors:  G D'Andrea; G Maurizi; A M D'Alessandro; M L Salucci; A Impagnatiello; M A Saletti; A Oratore
Journal:  J Protein Chem       Date:  1992-04

10.  Reliability of transferrin and leucine aminopeptidase phenotyping in wild meadow voles (Microtus pennsylvanicus).

Authors:  S Mihok; D Ewing
Journal:  Biochem Genet       Date:  1983-10       Impact factor: 1.890
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