Literature DB >> 7210604

Monoclonal anti-A from a hybrid-myeloma: evaluating as a blood grouping reagent.

D Voak, S Sacks, T Alderson, F Takei, E Lennox, J Jarvis, C Milstein, J Darnborough.   

Abstract

A monoclonal anti-A antibody has been evaluated and found suitable for use as a potent routine ABO grouping reagent, without the use of additives. The IgM anti-A (MH2/6D4) is secreted into the tissue culture supernatant by a permanent line of cloned cells derived by fusion of anti-A producing spleen cells and a mouse myeloma cell line. This is a cost-effective reagent which should reduce production costs by over 50%. This reagent has the advantages inherent in monoclonal antibodies among them the availability of unlimited quantities of unvarying antibody of known properties.

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Year:  1980        PMID: 7210604     DOI: 10.1111/j.1423-0410.1980.tb01848.x

Source DB:  PubMed          Journal:  Vox Sang        ISSN: 0042-9007            Impact factor:   2.144


  11 in total

Review 1.  Hybridomas: a new dimension in biological analyses.

Authors:  R H Kennett
Journal:  In Vitro       Date:  1981-12

2.  Variable localization of blood group antigen in group A kidneys.

Authors:  L P Trickett; P R Evans; A G MacIver; J L Smith; M Slapak
Journal:  Br J Exp Pathol       Date:  1983-04

3.  Evaluation of monoclonal anti-A and anti-B and affinity-purified Ulex europaeus lectin I for forensic blood grouping.

Authors:  R E Gaensslen; H C Lee; J E Carroll
Journal:  Z Rechtsmed       Date:  1984

4.  Thermodynamic and immunological properties of a monoclonal antibody to human blood group A.

Authors:  L Edelman; P Rouger; C Doinel; H Garchon; J F Bach; J Reviron; C Salmon
Journal:  Immunology       Date:  1981-11       Impact factor: 7.397

5.  A modified hybridoma technique for production of monoclonal antibodies having desired isotypes.

Authors:  Napaporn Apiratmateekul; Ponrat Phunpae; Watchara Kasinrerk
Journal:  Cytotechnology       Date:  2009-07-29       Impact factor: 2.058

6.  Quantitation of antibody uptake on A group erythrocytes using immunoautoradiography and monoclonal IgM anti-A.

Authors:  S C Gupte; E Thiel; O Majdic; S Thierfelder; H M Bhatia
Journal:  Blut       Date:  1985-10

7.  Human erythrocyte antigens. Regulation of expression of a novel erythrocyte surface antigen by the inhibitor Lutheran In(Lu) gene.

Authors:  M J Telen; G S Eisenbarth; B F Haynes
Journal:  J Clin Invest       Date:  1983-06       Impact factor: 14.808

8.  From the structure of antibodies to the diversification of the immune response.

Authors:  C Milstein
Journal:  EMBO J       Date:  1985-05       Impact factor: 11.598

9.  The British Association for Cancer Research, 23rd annual general meeting. Edinburgh 29--31 March 1982. Abstracts.

Authors: 
Journal:  Br J Cancer       Date:  1982-09       Impact factor: 7.640

10.  Blood group-active carbohydrate chains on the receptor for epidermal growth factor of A431 cells.

Authors:  R A Childs; M Gregoriou; P Scudder; S J Thorpe; A R Rees; T Feizi
Journal:  EMBO J       Date:  1984-10       Impact factor: 11.598

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