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Literature DB >> 7205995

A sensitive radioenzymatic assay for catechol drugs.

Abstract

This assay measures picogram quantities of catechol drugs and endogenous catecholamines in body tissues and fluids. The catechols are converted to their 3H-O-methyl metabolites during incubation with 3H-S-adenosylmethionine then separated by solvent extraction and thin-layer chromatography. Most drugs containing the catechol structure can be radiolabeled and separated from norepinephrine and epinephrine by this technique to provide simultaneous measurement of endogenous and exogenously administered catechols. The disposition of isoproterenol in tissues and fluids of man and experimental animals is measured to illustrate the utility of this assay. The reactivity of several commonly administered catechol drugs with COMT is described and the possible implications discussed.

Entities: Chemical Gene Species

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Year: 1980 PMID： 7205995 DOI： 10.1002/jnr.490050613

Source DB: PubMed Journal: J Neurosci Res ISSN： 0360-4012 Impact factor: 4.164

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Cited

13 in total

1. Differential effects of active and passive laboratory stressors on immune function in healthy men.

Authors: S G Zakowski; L Cohen; M H Hall; K Wollman; A Baum
Journal: Int J Behav Med Date: 1994

2. In vivo regulation of beta-adrenergic receptors on mononuclear leukocytes and heart. Assessment of receptor compartmentation after agonist infusion and acute aortic constriction in guinea pigs.

Authors: A S Maisel; M G Ziegler; S Carter; P A Insel; H J Motulsky
Journal: J Clin Invest Date: 1988-12 Impact factor: 14.808

10. Downregulation of cardiac guanosine 5'-triphosphate-binding proteins in right atrium and left ventricle in pacing-induced congestive heart failure.

Authors: D A Roth; K Urasawa; G A Helmer; H K Hammond
Journal: J Clin Invest Date: 1993-03 Impact factor: 14.808

A sensitive radioenzymatic assay for catechol drugs.

1. Differential effects of active and passive laboratory stressors on immune function in healthy men.

2. In vivo regulation of beta-adrenergic receptors on mononuclear leukocytes and heart. Assessment of receptor compartmentation after agonist infusion and acute aortic constriction in guinea pigs.

3. The effect of hemorrhage and resuscitation on serum levels of immunoreactive atrial natriuretic factor.

4. The effects of acute exercise on subsequent cigarette smoking.

5. Central cholinergic stimulation causes adrenal epinephrine release.

6. Changes in glomerular hemodynamic response to angiotensin II after subacute renal denervation in rats.

7. Functional effects on glomerular hemodynamics of short-term chronic cyclosporine in male rats.

8. Cardiovascular responses to electrical stimulation of sympathetic nerves in the pithed mouse.

9. A longitudinal evaluation of thyroid function in critically ill surgical patients.

10. Downregulation of cardiac guanosine 5'-triphosphate-binding proteins in right atrium and left ventricle in pacing-induced congestive heart failure.