Isolation and properties of sialodacryoadenitis virus of rats.

Pathogenic agents were isolated from affected salivary glands of rats with sialodacryoadenitis (SDA) by means of intercerebral inoculation to newborn mice and inoculation to primary rat kidney cell cultures. The properties of these isolates, irrespective of the isolation techniques, were in general agreement with that of SDA virus strain 681, and they were identified as SDA virus. In addition to the known properties, following characteristics were noticed: (1) Particles of SDA virus bore two different long and short surface projections. (2) Some antigenic differences were observed between the present isolate 930-10 and the strain 681. (3) It was suggested that SDA virus grew better at 35 degrees C than at 31 degrees, 33 degrees, 37 degrees or 39 degrees C. (4) SDA virus did not propagate in DBT cells.