Literature DB >> 7199976

Purification of tracer for somatomedin C radioimmunoassay by hydrophobic interaction chromatography.

R C Baxter, A S Brown.   

Abstract

We have purified tracer for use in the somatomedin C radioimmunoassay by hydrophobic interaction chromatography. Material showing greatest immunoreactivity binds to Octyl Sepharose CL-4B (Pharmacia) in a buffer mixture consisting of 130 mL of acetonitrile and 870 mL of 0.1 mol/L NH4HCO3, pH 7.8, but is eluted by increasing the acetonitrile content to 180 mL/L. As compared with tracer purified by binding to specific antiserum in liquid phase, precipitating the complex with second antibody, and then dissociating by gel chromatography at acid pH, this tracer shows equal immunoreactivity against specific somatomedin C antiserum. Either preparation allows excellent discrimination between extracts of normal, acromegalic, and hypopituitary plasma samples; thus either is suitable for use in the somatomedin C radioimmunoassay. Tracer purification by hydrophobic interaction chromatography is rapid and inexpensive. It may be useful in preparing highly immunoreactive tracers for other peptide radioimmunoassays.

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Year:  1982        PMID： 7199976

Source DB:  PubMed          Journal:  Clin Chem        ISSN： 0009-9147            Impact factor:   8.327


  8 in total

1.  Delineation of atypical insulin receptors from classical insulin and type I insulin-like growth factor receptors in human placenta.

Authors:  H A Jonas; A J Cox; L C Harrison
Journal:  Biochem J       Date:  1989-01-01       Impact factor: 3.857

2.  Structure of the Mr 140,000 growth hormone-dependent insulin-like growth factor binding protein complex: determination by reconstitution and affinity-labeling.

Authors:  R C Baxter; J L Martin
Journal:  Proc Natl Acad Sci U S A       Date:  1989-09       Impact factor: 11.205

3.  Monoclonal antibody to the type I insulin-like growth factor (IGF-I) receptor blocks IGF-I receptor-mediated DNA synthesis: clarification of the mitogenic mechanisms of IGF-I and insulin in human skin fibroblasts.

Authors:  J S Flier; P Usher; A C Moses
Journal:  Proc Natl Acad Sci U S A       Date:  1986-02       Impact factor: 11.205

4.  An atypical insulin receptor with high affinity for insulin-like growth factors copurified with placental insulin receptors.

Authors:  H A Jonas; J D Newman; L C Harrison
Journal:  Proc Natl Acad Sci U S A       Date:  1986-06       Impact factor: 11.205

5.  Disulphide reduction alters the immunoreactivity and increases the affinity of insulin-like growth-factor-I receptors in human placenta.

Authors:  H A Jonas; L C Harrison
Journal:  Biochem J       Date:  1986-06-01       Impact factor: 3.857

6.  Glycosaminoglycans inhibit formation of the 140 kDa insulin-like growth factor-binding protein complex.

Authors:  R C Baxter
Journal:  Biochem J       Date:  1990-11-01       Impact factor: 3.857

7.  Insulin-like growth factor-1, growth hormone-dependent insulin-like growth factor-binding protein and growth in children with chronic renal failure.

Authors:  E M Hodson; A S Brown; L P Roy; A R Rosenberg
Journal:  Pediatr Nephrol       Date:  1992-09       Impact factor: 3.714

8.  Comparative binding of bovine, human and rat insulin-like growth factors to membrane receptors and to antibodies against human insulin-like growth factor-1.

Authors:  L C Read; F J Ballard; G L Francis; R C Baxter; C J Bagley; J C Wallace
Journal:  Biochem J       Date:  1986-01-01       Impact factor: 3.857
  8 in total

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