Literature DB >> 7199537

Pitfalls in the somatomedin-C radioimmunoassay.

R W Furlanetto.   

Abstract

We have investigated the effects of various modifications on the quantitation of Somatomedin-C (Sm-C) by the Sm-C radio immunoassay. We have confirmed that the assay quantitatively measures Sm-C in serum at plasma samples when borosilicate tubes and protamine free buffers are used. Polystyrene tubes and/or protamine containing buffers interfere with the quantitative measurement of the hormone, apparently by modifying the binding of Sm-C to its binding protein. The implication of these modifications on the clinical use of the Sm-C RIA are discussed.

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Year:  1982        PMID： 7199537     DOI： 10.1210/jcem-54-5-1084

Source DB:  PubMed          Journal:  J Clin Endocrinol Metab        ISSN： 0021-972X            Impact factor:   5.958


  9 in total

1.  Demonstration of the production and physiological role of insulin-like growth factor II in rat thyroid follicular cells in culture.

Authors:  R M Maciel; A C Moses; G Villone; D Tramontano; S H Ingbar
Journal:  J Clin Invest       Date:  1988-11       Impact factor: 14.808

2.  Pulsatile growth hormone secretion in normal man during a continuous 24-hour infusion of human growth hormone releasing factor (1-40). Evidence for intermittent somatostatin secretion.

Authors:  M L Vance; D L Kaiser; W S Evans; R Furlanetto; W Vale; J Rivier; M O Thorner
Journal:  J Clin Invest       Date:  1985-05       Impact factor: 14.808

3.  Somatomedin-C levels related to gestational age, birth weight and day of life.

Authors:  A Cassio; M Capelli; E Cacciari; A Cicognani; P Pirazzoli; F Righetti; D Ballardini; G Natali; S Zucchini; E Martelli
Journal:  Eur J Pediatr       Date:  1986-08       Impact factor: 3.183

4.  Serum insulin-like growth factor-I levels in amyotrophic lateral sclerosis.

Authors:  G D Braunstein; A L Reviczky
Journal:  J Neurol Neurosurg Psychiatry       Date:  1987-06       Impact factor: 10.154

5.  Growth and growth factors in diabetes mellitus.

Authors:  S Salardi; S Tonioli; P Tassoni; M Tellarini; L Mazzanti; E Cacciari
Journal:  Arch Dis Child       Date:  1987-01       Impact factor: 3.791

6.  Insulin-like growth factor I/somatomedin C: a potent inducer of oligodendrocyte development.

Authors:  F A McMorris; T M Smith; S DeSalvo; R W Furlanetto
Journal:  Proc Natl Acad Sci U S A       Date:  1986-02       Impact factor: 11.205

7.  Insulin-like growth factor I: a good indicator of functional hepatocellular capacity in alcoholic liver cirrhosis.

Authors:  A Caufriez; P Reding; D Urbain; J Golstein; G Copinschi
Journal:  J Endocrinol Invest       Date:  1991-04       Impact factor: 4.256

8.  Insulin-Like Growth Factor I in Human Thyroid Tissue: Specific Localization by Immunohistochemistry and In Situ Hybridization.

Authors:  Rui M. B. Maciel; Edna T. Kimura; Mirian H. Takahaski; Maria-Honorina C. Lopes; Maria-Isabel S. Mesquita; Alan C. Moses; Vania N. Alberti
Journal:  Endocr Pathol       Date:  1995       Impact factor: 3.943

9.  Immunodetection of Insulin-Like Growth Factor I (IGF-l) in Normal and Pathological Adrenocortical Tissue.

Authors:  Samir Faical; Rui M. B. Maciel; Vania Nose-Alberti; Marivania C. Santos; Claudio E. Kater
Journal:  Endocr Pathol       Date:  1998       Impact factor: 3.943
  9 in total

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