Literature DB >> 7199

Cultivation of mycoplasmas in a modified tissue culture medium.

M G Gabridge, S E Singer, R A Esposito.   

Abstract

A new medium, which contained a chemically defined tissue culture base ("medium 199"), was developed for the cultivation of mycoplasmas. When supplemented with albumin, glucose, serum, and yeast extract, the new medium adequately supported the growth of Mycoplasma and Acholeplasma species.

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Year:  1976        PMID： 7199      PMCID： PMC169865          DOI： 10.1128/aem.31.6.986-989.1976

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  16 in total

1.  Oxygen consumption by trachea organ cultures infected with Mycoplasma pneumoniae.

Authors:  M G Gabridge
Journal:  Infect Immun       Date:  1975-09       Impact factor: 3.441

2.  Nuclease activities of Mycoplasma gallisepticum as a function of culture age in different media.

Authors:  B S Cowen; S C Smith
Journal:  J Bacteriol       Date:  1972-01       Impact factor: 3.490

3.  Growth of parasitic Mycoplasma without serum of serum fraction.

Authors:  H Kurzepa; L Flinton; P J VanDemark
Journal:  J Bacteriol       Date:  1969-09       Impact factor: 3.490

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Some effects of growth medium composition on the antigenicity of a T-strain mycoplasma.

Authors:  G K Masover; R P Mischak; L Hayflick
Journal:  Infect Immun       Date:  1975-03       Impact factor: 3.441

6.  The influence of pH of the culture medium on the sensitivity of Mycoplasma gallisepticum antigens for use in certain serological tests.

Authors:  J M Bradbury; F T Jordan
Journal:  J Hyg (Lond)       Date:  1971-12

7.  Quantitative reduction of 2,3,4-triphenyl tetrazolium chloride by hamster trachea organ cultures: effects of Mycoplasma pneumoniae cells and membranes.

Authors:  M G Gabridge; R B Polisky
Journal:  Infect Immun       Date:  1976-01       Impact factor: 3.441

8.  Cultivation of mycoplasmas on cellulose ester substrates.

Authors:  M G Gabridge
Journal:  Appl Microbiol       Date:  1973-09

9.  Differences in arginine requirement for growth among arginine-utilizing Mycoplasma species.

Authors:  R G Hahn; G E Kenny
Journal:  J Bacteriol       Date:  1974-02       Impact factor: 3.490

10.  Influence of lipid components of Mycoplasma laidlawii membranes on osmotic fragility of cells.

Authors:  S Razin; M E Tourtellotte; R N McElhaney; J D Pollack
Journal:  J Bacteriol       Date:  1966-02       Impact factor: 3.490
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  11 in total

1.  Isotype commitment of B cells and dissemination of the primed state after mucosal stimulation with Mycoplasma pulmonis.

Authors:  F V Rose; J J Cebra
Journal:  Infect Immun       Date:  1985-08       Impact factor: 3.441

2.  Differences in the attachment of Mycoplasma pneumoniae cells and membranes to tracheal epithelium.

Authors:  M G Gabridge; Y D Barden-Stahl; R B Polisky; J A Engelhardt
Journal:  Infect Immun       Date:  1977-06       Impact factor: 3.441

3.  Role of cell-associated pathogen metabolism in infection of tracheal explants by Mycoplasma pneumoniae.

Authors:  M G Gabridge; Y Dee Barden Stahl
Journal:  Med Microbiol Immunol       Date:  1978-10-20       Impact factor: 3.402

4.  Interaction of Mycoplasma pneumoniae with human lung fibroblasts: characterization of the in vitro model.

Authors:  M G Gabridge; D Taylor-Robinson; H A Davies; R R Dourmashkin
Journal:  Infect Immun       Date:  1979-07       Impact factor: 3.441

5.  Effect of in vitro cultivation and Mycoplasma pneumoniae infection on intracellular cyclic AMP levels in hamster tracheal organ cultures.

Authors:  B K Wolffing; M G Gabridge
Journal:  In Vitro       Date:  1979-04

6.  Role of host cell metabolism in the pathogenesis of Mycoplasma pneumoniae infection.

Authors:  S Upchurch; M G Gabridge
Journal:  Infect Immun       Date:  1981-01       Impact factor: 3.441

7.  Intracellular levels of adenosine triphosphate in hamster trachea organ cultures exposed to Mycoplasma pneumoniae cells or membranes.

Authors:  M G Gabridge; R B Polisky
Journal:  In Vitro       Date:  1977-08

8.  Role of adenine in pathogenesis of Mycoplasma pneumoniae infections of tracheal epithelium.

Authors:  M G Gabridge; Y D Stahl
Journal:  Med Microbiol Immunol       Date:  1978-05-26       Impact factor: 3.402

9.  Scanning electron microscopy of Mycoplasma pneumoniae on the membrane of individual ciliated tracheal cells.

Authors:  M G Gabridge; M J Bright; H R Richards
Journal:  In Vitro       Date:  1982-01

10.  Antimycoplasmal activity of dimethylphenols in a tracheal explant culture system.

Authors:  C C Agee; J A Engelhardt; M G Gabridge
Journal:  Antimicrob Agents Chemother       Date:  1980-08       Impact factor: 5.191
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