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Literature DB >> 7196901

Histochemical localization of nitrate reductase.

Abstract

NADH-dependent nitrate reductase (E.C. 1.6.6.1) was ultrastructurally localized in norflurazon-treated and control soybean cotyledons [Glycine max (L.) Merr.] by a method based upon the increase in osmiophilia due to the formation of an azo dye. The reaction product was observed in small vesicles throughout the cytoplasm. An apparent transport of nitrite to the plastid, the site of nitrite reduction, may occur through fusion of the nitrite-containing vesicles with the chloroplast envelope. Plants grown in tungstate lacked nitrate reductase activity as measured by standard assay procedures, and showed no increase in osmiophilia, suggesting a degree of specificity of this cytochemical procedure.

Entities: Chemical Gene Species

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Year: 1981 PMID： 7196901 DOI： 10.1007/bf00517132

Source DB: PubMed Journal: Histochemistry ISSN： 0301-5564

5 in total

1. Studies on the sub-cellular location of particulate nitrate and nitrite reductase, glutamic dehydrogenase and other enzymes in barley roots.

Authors: B J Miflin
Journal: Planta Date: 1970-06 Impact factor: 4.116

2. Generation of reduced nicotinamide adenine dinucleotide for nitrate reduction in green leaves.

Authors: L Klepper; D Flesher; R H Hageman
Journal: Plant Physiol Date: 1971-11 Impact factor: 8.340

3. Evidence for a role of calcium in nitrate assimilation in wheat seedlings.

Authors: G M Paulsen; J E Harper
Journal: Plant Physiol Date: 1968-05 Impact factor: 8.340

4. Intracellular location of nitrate reductase and nitrite reductase in spinach and sunflower leaves.

Authors: B R Grant; C A Atkins; D T Canvin
Journal: Planta Date: 1970-03 Impact factor: 4.116

5. An ultrastructural study of acid phosphatase localization in Phaseolus vulgaris xylem by the use of an azo-dye method.

Authors: I Charvat; K Esau
Journal: J Cell Sci Date: 1975-12 Impact factor: 5.285

5 in total

9 in total

6. Carbon dioxide and nitrite photoassimilatory processes do not intercompete for reducing equivalents in spinach and soybean leaf chloroplasts.

Authors: J M Robinson
Journal: Plant Physiol Date: 1986-03 Impact factor: 8.340

Histochemical localization of nitrate reductase.

1. Studies on the sub-cellular location of particulate nitrate and nitrite reductase, glutamic dehydrogenase and other enzymes in barley roots.

2. Generation of reduced nicotinamide adenine dinucleotide for nitrate reduction in green leaves.

3. Evidence for a role of calcium in nitrate assimilation in wheat seedlings.

4. Intracellular location of nitrate reductase and nitrite reductase in spinach and sunflower leaves.

5. An ultrastructural study of acid phosphatase localization in Phaseolus vulgaris xylem by the use of an azo-dye method.

1. Nitrate reductase of green algae is located in the pyrenoid.

2. Localization of Nitrogen-Assimilating Enzymes in the Chloroplast of Chlamydomonas reinhardtii.

3. Blue Light, a Positive Switch Signal for Nitrate and Nitrite Uptake by the Green Alga Monoraphidium braunii.

4. Immunogold localization of nitrate reductase in maize leaves.

5. Nitrate Reductase from Monoraphidium braunii: Immunocytochemical Localization and Immunological Characterization.

6. Carbon dioxide and nitrite photoassimilatory processes do not intercompete for reducing equivalents in spinach and soybean leaf chloroplasts.

7. Differential light induction of nitrate reductases in greening and photobleached soybean seedlings.

8. Characterization of the association of nitrate reductase with barley (Hordeum vulgare L.) root membranes.

9. The effect of oxygen on nitrate and nitrite assimilation in leaves of Zea mays L. under dark conditions.