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Literature DB >> 7195557

A liquid ion-exchanger alternative to KCl for filling intracellular reference microelectrodes.

Abstract

We have developed a filling solution for silanised microelectrodes consisting of potassium tetrakis (p-chlorophenyl) borate in octanol. Microelectrodes filled with this reference liquid ion-exchanger (RLIE) have equal selectivities to Na and K, and give the same membrane potential as do KCl-filled microelectrodes. The RLIE microelectrodes are more stable, less damaging to the cell membrane and do not leak Cl- ions. Their high resistance, however, makes them unsuitable for recording rapid potential changes or for passing current.

Entities: Chemical

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Year: 1981 PMID： 7195557 DOI： 10.1007/bf00582719

Source DB: PubMed Journal: Pflugers Arch ISSN： 0031-6768 Impact factor: 3.657

8 in total

1. The specific ionic conductances and the ionic movements across the motoneuronal membrane that produce the inhibitory post-synaptic potential.

Authors: J S COOMBS; J C ECCLES; P FATT
Journal: J Physiol Date: 1955-11-28 Impact factor: 5.182

2. The effect of internal and external potassium concentration on the membrane potential of frog muscle.

Authors: R H ADRIAN
Journal: J Physiol Date: 1956-09-27 Impact factor: 5.182

3. The normal membrane potential of frog sartorius fibers.

Authors: G LING; R W GERARD
Journal: J Cell Comp Physiol Date: 1949-12

4. A method of making fine double-barrelled potassium-sensitive micro-electrodes for intracellular recording [proceedings].

Authors: J A Coles; M Tsacopoulos
Journal: J Physiol Date: 1977-08 Impact factor: 5.182

25 in total

Review 4. Investigation of factors affecting the intracellular sodium activity in the smooth muscle of guinea-pig ureter.

Authors: C C Aickin
Journal: J Physiol Date: 1987-04 Impact factor: 5.182

A liquid ion-exchanger alternative to KCl for filling intracellular reference microelectrodes.

1. The specific ionic conductances and the ionic movements across the motoneuronal membrane that produce the inhibitory post-synaptic potential.

2. The effect of internal and external potassium concentration on the membrane potential of frog muscle.

3. The normal membrane potential of frog sartorius fibers.

4. A method of making fine double-barrelled potassium-sensitive micro-electrodes for intracellular recording [proceedings].

5. The role of bicarbonate, chloride and sodium ions in the regulation of intracellular pH in snail neurones.

6. Some problems involved in electric measurements of biological systems.

7. Neutral carrier ion-selective microelectrodes for measurement of intracellular free calcium.

8. n-Alkanols potentiate sodium channel inactivation in squid giant axons.

1. Evidence against a contribution by Na(+)-Cl- cotransport to chloride accumulation in rat arterial smooth muscle.

2. A near-zero membrane potential in transporting corneal endothelial cells of rabbit.

3. Apical membrane potassium and chloride permeabilities in surface cells of rabbit descending colon epithelium.

Review 4. Investigation of factors affecting the intracellular sodium activity in the smooth muscle of guinea-pig ureter.

5. Cell membrane and transepithelial voltages and resistances in isolated rat hepatocyte couplets.

6. The role of chloride-bicarbonate exchange in the regulation of intracellular chloride in guinea-pig vas deferens.

7. Direct measurement of intracellular pH and buffering power in smooth muscle cells of guinea-pig vas deferens.

8. Intracellular chloride and the mechanism for its accumulation in rat lumbrical muscle.

9. Acid influx into snail neurones caused by reversal of the normal pHi-regulating system.

10. Towards an estimate of chloride permeability in the smooth muscle of guinea-pig vas deferens.