[Immunization against Capillaria hepatica: the effects of primary infections, x-irradiated stages, non-embryonated eggs, and soluble egg extracts (author's transl)].

The influence of primary infections with embryonated infective eggs or with X-irradiated infective eggs, and of non-embryonated eggs, and egg homogenate extracts on challenge infections with Capillaria hepatica was investigated. The worm reproductivity was significantly suppressed in a sublethal challenge infection given 11 days after a primary infection of Mastomys natalensis with 50, 150, 400, and 800 eggs per animal. The administration of 600 X-irradiated (2.2 Krd) embryonated eggs 36 days before challenge as well as an intraperitoneal injection of non-embryonated eggs 12, 10, 8, 6, 4, and 2 days before challenge (simulating the egg production of a normal infection) also reduced significantly the egg production of a weak (50 eggs/ animal) infection. No effect was observed on a moderate challenge (300 eggs/animal). The effect was not markedly enhanced by the repeated administration of X-irradiated eggs or by the combination of X-irradiated infective eggs and non-embryonated eggs. Immunization of mice with soluble egg extracts resulted in significant reduction of egg production determined 60 days after challenge. Two hundred and thirty eggs of C. hepatica/g body weight proved to be a lethal infection dose for M. natalensis. The animals died between 20 and 35 days after infection. After single infections with 50, 150, 400, or 800 eggs per animal the mortality of Mastomys challenged 36 or 52 days later was reduced to 0--30%. Using X-irradiated embryonated eggs for immunization only repeated administration led to protection in 70 to 80% of the animals. About 40% of the animals could be protected by the intraperitoneal injection of non-embryonated eggs. If death occurred it was delayed. The combination of X-irradiated stages and eggs did not enhance the protection.