Literature DB >> 7191374

Specificity in the hydrolysis of N-acyl-L-phenylalanine 4-nitroanilides by chymotrypsin.

H D Jakubke, H Däumer, A Könnecke, P Kuhl, J Fischer.   

Abstract

The affinity of N-acyl-L-phenylalanine 4-nitroanilides for chymotrypsin is enhanced as the hydrophobicity of non-amino acid residues in the P2-position of the substrates increases, whereas Kcat remains nearly constant. On the other hand, if alanine or leucine is in the P2-position Kcat increases with decreasing KM.

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Year:  1980        PMID： 7191374     DOI： 10.1007/bf01965954

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  14 in total

Review 1.  MECHANISM OF ACTION OF PROTEOLYTIC ENZYMES.

Authors:  M L BENDER; J KEZDY
Journal:  Annu Rev Biochem       Date:  1965       Impact factor: 23.643

2.  Substrate induced pK perturbations with chymotrypsin and the possible significance of nonproductive binding.

Authors:  B Zeeberg; M Caplow; M Caswell
Journal:  J Am Chem Soc       Date:  1975-12-10       Impact factor: 15.419

3.  Three-dimensional structure of tosyl-alpha-chymotrypsin.

Authors:  B W Matthews; P B Sigler; R Henderson; D M Blow
Journal:  Nature       Date:  1967-05-13       Impact factor: 49.962

4.  Subsite interactions in chymotrypsin as reflected by acyl enzyme stability.

Authors:  M J Moffitt; G E Means
Journal:  Biochem Biophys Res Commun       Date:  1978-08-29       Impact factor: 3.575

5.  Kinetics of the reaction of chymotrypsin A with peptide chloromethyl ketones in relation to its subsite specificity.

Authors:  K Kurachi; J C Powers; P E Wilcox
Journal:  Biochemistry       Date:  1973-02       Impact factor: 3.162

6.  Substrate binding site in bovine chymotrypsin A-gamma. A crystallographic study using peptide chloromethyl ketones as site-specific inhibitors.

Authors:  D M Segal; J C Powers; G H Cohen; D R Davies; P E Wilcox
Journal:  Biochemistry       Date:  1971-09-28       Impact factor: 3.162

7.  The determination of the concentration of hydrolytic enzyme solutions: alpha-chymotrypsin, trypsin, papain, elastase, subtilisin, and acetylcholinesterase.

Authors:  M L Bender; M L Begué-Cantón; R L Blakeley; L J Brubacher; J Feder; C R Gunter; F J Kézdy; J V Killheffer; T H Marshall; C G Miller; R W Roeske; J K Stoops
Journal:  J Am Chem Soc       Date:  1966-12-20       Impact factor: 15.419

8.  On the size of the active site in proteases. I. Papain.

Authors:  I Schechter; A Berger
Journal:  Biochem Biophys Res Commun       Date:  1967-04-20       Impact factor: 3.575

9.  Structural specificity of alpha-chymotrypsin: polypeptide substrates.

Authors:  G L Neil; C Niemann; G E Hein
Journal:  Nature       Date:  1966-05-28       Impact factor: 49.962

10.  Catalysis and leaving group binding in anilide hydrolysis by chymotrypsin.

Authors:  D Petkov; E Christova; I Stoineva
Journal:  Biochim Biophys Acta       Date:  1978-11-10
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