Literature DB >> 7190739

Formation of hybridoma clones in soft agarose: effect of pH and of medium.

J Sharon, S L Morrison, E A Kabat.   

Abstract

Optimal conditions for the formation of hybridoma clones in soft agarose are described. The hybridization frequency is shown to be highly dependent on the pH of the polyethylene glycol (PEG) solution used for fusion and on the cloning medium. Maximal numbers of clones are obtained when the PEG solution used for fusion is at pH 8.0-8.2.

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Year:  1980        PMID： 7190739     DOI： 10.1007/bf01542794

Source DB:  PubMed          Journal:  Somatic Cell Genet        ISSN： 0098-0366


  4 in total

1.  Somatic cell cloning in polyester stacks.

Authors:  C R Raetz; M M Wermuth; T M McIntyre; J D Esko; D C Wing
Journal:  Proc Natl Acad Sci U S A       Date:  1982-05       Impact factor: 11.205

2.  Production of monoclonal antibodies to staphylococcal enterotoxin A.

Authors:  C Edwin; S R Tatini; R S Strobel; S K Maheswaran
Journal:  Appl Environ Microbiol       Date:  1984-12       Impact factor: 4.792

3.  Diagnosis of cutaneous T cell lymphoma by use of monoclonal antibodies reactive with tumor-associated antigens.

Authors:  C L Berger; S Morrison; A Chu; J Patterson; A Estabrook; S Takezaki; J Sharon; D Warburton; O Irigoyen; R L Edelson
Journal:  J Clin Invest       Date:  1982-12       Impact factor: 14.808

4.  Generation of functional human T cell hybrids.

Authors:  O Irigoyen; P V Rizzolo; Y Thomas; L Rogozinski; L Chess
Journal:  J Exp Med       Date:  1981-12-01       Impact factor: 14.307
  4 in total

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