| Literature DB >> 7188739 |
D S Ithakissios, D O Kubiatowicz, J H Wicks.
Abstract
We describe a vitamin B12 radioassay with oyster toadfish (Opsanus tau) serum in which endogenous serum B12 is released from its protein complexes at room temperature with NaOH at pH 13.6. The radioassay is reproducible (CV 4.9% within assay, 8.4% between assays) and sensitive (10 ng/L) and requires minimum manipulations. Assay accuracy is unaffected by serum protein.Entities:
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Year: 1980 PMID： 7188739
Source DB: PubMed Journal: Clin Chem ISSN： 0009-9147 Impact factor: 8.327