Membrane potential and contractures in segments cut from rat fast and slow twitch muscles.

Contractile responses associated with depolarization caused by an increase in [K]0 or by voltage-clamp steps were compared for fast and slow mammalian twitch muscles. The contractions and contractures of isolated mammalian muscle fibres cut into 0.5-1.0 cm lengths are similar to those obtained from intact cells. The slope of the relationship between the membrane potential and the log [K]0 is similar in slow (46 mV +/- 0.8) and in fast fibres (48 mV +/- 1.1). This relation is not significantly modified in sodium-free or Cl-free solution. The K-contractures of cut sections of slow and fast fibres are transient and a full repriming of the response is only observed when the [K] x [Cl] product is kept constant. The contractile threshold in fast fibres is at 20-30 mM [K]0 (-52 to -43 mV) and in slow muscle at 10-15 mM [K]0 (-62 to -55 mV). Under voltage-clamp conditions, the contractile responses of both types of muscle show two components. In Na-free solution or in presence of TTX (5 x 10(-7) g/ml) the first component is abolished and the second remaining component is similar to that developing during K-contractures. In iliacus fibres, the contracture threshold is between -50.5 mV and -40.5 mV and in soleus fibres between -66 mV and -56 mV; these values are close to those obtained with K-rich depolarizing fluids. The S-shaped curve of the contracture vs membrane potential relation is similar to that found in frog muscles except that the contractile responses are graded over a larger range of membrane potentials (-50 to + 30 mV in fast and -55 to + 10 mV in slow muscle).