Literature DB >> 7176822

Isolation of proline and hydroxyproline from fossil bone.

T W Stafford, R C Duhamel, C V Haynes, K Brendel.   

Abstract

We describe a simple and rapid procedure for the isolation of proline and hydroxyproline from fossil bone based on the isolation of gelatin, hydrolysis, purification with XAD-2, deamination of primary amino acids with aqua regia, and separation of the imino acids by cation exchange chromatography. This procedure will provide material for accurate bone carbon dating, and stable carbon isotope ratio determinations for the evaluation of paleodiets.

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Year:  1982        PMID: 7176822     DOI: 10.1016/0024-3205(82)90551-3

Source DB:  PubMed          Journal:  Life Sci        ISSN: 0024-3205            Impact factor:   5.037


  2 in total

1.  Single amino acid radiocarbon dating of Upper Paleolithic modern humans.

Authors:  Anat Marom; James S O McCullagh; Thomas F G Higham; Andrey A Sinitsyn; Robert E M Hedges
Journal:  Proc Natl Acad Sci U S A       Date:  2012-04-18       Impact factor: 11.205

2.  The timing and effect of the earliest human arrivals in North America.

Authors:  Lorena Becerra-Valdivia; Thomas Higham
Journal:  Nature       Date:  2020-07-22       Impact factor: 49.962

  2 in total

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