Two different anti-galactan lectins in eel serum.

Two different anti-galactan lectins from eel serum (Anguilla anguilla) were isolated by affinity chromatography on fucogel and on 1-acyl-2-(9-carboxy)nonyl-glycero-3-phosphocholine coupled to Aminohexyl-Sepharose. The fucolectin and the CRP-analogue protein thus purified directly from the eel serum were characterized by SDS-gradient gel electrophoresis, immunoelectrophoresis, and by agar gel diffusion tests. The CRP-analogue protein with a molecular weight of 24.800 Daltons was present in a high concentration. Carbohydrates are absent in both the proteins, showing that the fucolectin and the CRP analogue do not belong to the immunoglobulin/antibody group of serum proteins.