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Literature DB >> 717182

Regional levels of histamine in rat brain after microwave irradiation: evidence for artifacts in the enzymatic--isotopic assay.

N Subramanian, W Schinzel, P Mitznegg, C J Estler.

Abstract

Histamine levels in the brain, measured fluorimetrically, were similar for rats killed by decapitation and microwave irradiation. However, enzymatic--isotopic assay yields 5--20 times higher value for microwave irradiation versus decapitation. Thin layer chromatography of the methylated products in the enzymatic--isotopic assay indicated the formation of artifacts following microwave irradiation. The results strongly suggest that a combination of microwave irradiation and enzymatic--isotopic assay is not suitable for histamine analysis in the rat brain.

Entities: Chemical Species

Mesh：

Substances：
Histamine

Year: 1978 PMID： 717182 DOI： 10.1007/bf02111434

Source DB: PubMed Journal: Agents Actions ISSN： 0065-4299

12 in total

1. Evaluation of the necessity for rapid inactivation of brain enzymes prior to analysis of norepinephrine dopamine and serotonin in the mouse.

Authors: S T Weintraub; W B Stavinoha; R L Pike; W W Morgan; A T Modak; S H Koslow; L Blank
Journal: Life Sci Date: 1975-11-01 Impact factor: 5.037

2. Regional levels of cyclic AMP in rat brain: pitfalls of microwave inactivation.

Authors: R H Lenox; J L Meyerhoff; O P Gandhi; H L Wray
Journal: J Cyclic Nucleotide Res Date: 1977-10

3. Microwave irradiation and brain gamma-aminobutyric acid levels in mice.

Authors: D L Richardson; C L Scudder
Journal: Life Sci Date: 1976-06-15 Impact factor: 5.037

4. Elevation in rat brain histamine content after focused microwave irradiation.

Authors: L B Hough; E F Domino
Journal: J Neurochem Date: 1977-08 Impact factor: 5.372

5. Effects of prostaglandins E1, E2 and F2a on cyclic AMP levels in brain in vivo.

Authors: W Wellmann; U Schwabe
Journal: Brain Res Date: 1973-09-14 Impact factor: 3.252

6. Isotopic microassay of histamine, histidine, histidine decarboxylase and histamine methyltransferase in brain tissue.

Authors: K M Taylor; S H Snyder
Journal: J Neurochem Date: 1972-05 Impact factor: 5.372

7. Use of microwave irradiation to prevent postmortem catecholamine metabolism: evidence for tissue disruption artifact in a discrete region of rat brain.

Authors: N S Sharpless; L L Brown
Journal: Brain Res Date: 1978-01-20 Impact factor: 3.252

8. Focussed microwave radiation: a technique to minimize post mortem changes of cyclic nucleotides, dopa and choline and to preserve brain morphology.

Authors: A Guidotti; D L Cheney; M Trabucchi; M Doteuchi; C Wang
Journal: Neuropharmacology Date: 1974-12 Impact factor: 5.250

9. A sensitive and specific method for the determination of histomine in human whole blood and plasma.

Authors: W Lorenz; H J Reimann; H Barth; J Kusche; R Meyer; A Doenicke; M Hutzel
Journal: Hoppe Seylers Z Physiol Chem Date: 1972-06

10. Hypothalamic 24-hour rhythms in histamine, histidine, decarboxylase and histamine-N-methyltransferase.

Authors: E Orr; W B Quay
Journal: Endocrinology Date: 1975-04 Impact factor: 4.736

4 in total

1. Histamine and peptic ulcer: influence of sample-taking on the precision and accuracy of fluorometric histamine assay in biopsies of human gastric mucosa.

Authors: H Rhode; W Lorenz; H Troidl; H J Reimann; G Häfner; D Weber
Journal: Agents Actions Date: 1980-04