Literature DB >> 7161151

Lipid extracting properties of 2,2-dimethoxypropane as revealed by electron microscopy and thin layer chromatography.

H J Beckmann, R Dierichs.   

Abstract

Dehydration with 2,2-dimethoxypropane acidified with either 1 N HCL or 0.05 N HCL in an 1:100 ratio has been found to extract various biologically active lipids. The effect is enhanced by an increased proton concentration and seems to occur only after complete H2O transformation. It is suggested that lipid extraction is caused by a reoxidation of lower osmium oxides with subsequent attack on the unfixed lipids by the organic solvent DMP.

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Year:  1982        PMID: 7161151     DOI: 10.1007/bf00543960

Source DB:  PubMed          Journal:  Histochemistry        ISSN: 0301-5564


  3 in total

1.  Rapid chemical dehydration of samples for electron microscopic examinations.

Authors:  L L Muller; T J Jacks
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2.  Optimization and investigation of the use of 2,2-dimethoxypropane as a dehydration agent for plant tissues in transmission electron microscopy.

Authors:  J R Thorpe; D M Harvey
Journal:  J Ultrastruct Res       Date:  1979-08

3.  Problems of the use of 2,2-dimethoxypropane as dehydrating agent in preparing single cells for transmission electron microscopy.

Authors:  R Dierichs; C Dosche
Journal:  Histochemistry       Date:  1982
  3 in total
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Authors:  Inna Sabdyusheva Litschauer; Klaus Becker; Saiedeh Saghafi; Simone Ballke; Christine Bollwein; Meraaj Foroughipour; Julia Gaugeler; Massih Foroughipour; Viktória Schavelová; Viktória László; Balazs Döme; Christine Brostjan; Wilko Weichert; Hans-Ulrich Dodt
Journal:  Sci Rep       Date:  2020-10-19       Impact factor: 4.379

  2 in total

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