Literature DB >> 7160540

Quantitation of glycosylated hemoglobin. Elimination of labile glycohemoglobin during sample hemolysis at pH 5.

E Bissé, W Berger, R Flückiger.   

Abstract

A simple method for the elimination of labile glycohemoglobin in the chromatographic quantitation of glycosylated hemoglobin is described. Use is made of the instability of Schiff base adducts in acidic solution. Erythrocytes are lysed with a pH 5 buffer. At this pH dissociation reaches completion during sample preparation.

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Year:  1982        PMID: 7160540     DOI: 10.2337/diab.31.7.630

Source DB:  PubMed          Journal:  Diabetes        ISSN: 0012-1797            Impact factor:   9.461


  7 in total

1.  Seasonal variation of haemoglobin A1 in children with insulin-dependent diabetes mellitus.

Authors:  F R Hinde; P J Standen; N P Mann; D I Johnston
Journal:  Eur J Pediatr       Date:  1989-06       Impact factor: 3.183

Review 2.  [The effect of Maillard reaction products on enzyme reactions].

Authors:  D Schumacher; L W Kroh
Journal:  Z Ernahrungswiss       Date:  1996-09

3.  Some factors that influence the nonenzymatic glycation of peptides and polypeptides by glyceraldehyde.

Authors:  Y Bai; H Ueno; J M Manning
Journal:  J Protein Chem       Date:  1989-04

Review 4.  Glycosylated haemoglobin: measurement and clinical use.

Authors:  I Peacock
Journal:  J Clin Pathol       Date:  1984-08       Impact factor: 3.411

5.  The measurement of hemoglobin A1. Evaluation of an 'aldimine eliminator'.

Authors:  J Ditzel; T Jensen
Journal:  Acta Diabetol Lat       Date:  1984 Apr-Jun

6.  A novel method for generating region-specific monoclonal antibodies to modified proteins. Application to the identification of human glucosylated low density lipoproteins.

Authors:  L K Curtiss; J L Witztum
Journal:  J Clin Invest       Date:  1983-10       Impact factor: 14.808

7.  Glycation of monoclonal antibodies impairs their ability to bind antigen.

Authors:  D M Kennedy; A W Skillen; C H Self
Journal:  Clin Exp Immunol       Date:  1994-11       Impact factor: 4.330

  7 in total

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