| Literature DB >> 7153453 |
G L Lookhart, B L Jones, D B Cooper, S B Hall.
Abstract
A method is described for quantitatively hydrolyzing proteins in 45 min and for analyzing the hydrolysates by high-performance liquid chromatography in an additional 52 min. The alpha-amino acids were detected by the fluorescence of their omicron-phthaldialdehyde derivatives. Ten picomoles of each of the commonly occurring alpha-amino acids could be reliably determined. The method described yielded OPA-ethanethiolamino acid derivatives that were stable for 1 1/2 h and the HPLC method produced a better separation than previously published methods.Entities:
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Year: 1982 PMID： 7153453 DOI： 10.1016/0165-022x(82)90032-x
Source DB: PubMed Journal: J Biochem Biophys Methods ISSN： 0165-022X