| Literature DB >> 7150646 |
S Ramakrishnan, M R Eagle, L L Houston.
Abstract
A radioimmunoassay for ricin and ricin A- and B-chains was developed. Amounts as low as 100 pg of A-chain and 500 pg of B-chain could easily be quantitated. We showed, however, that the free chains were more reactive in the radioimmunoassay than the equivalent quantity of the individual chains when combined in intact ricin. The usefulness of the assay was demonstrated by determining the concentration of contaminating A- or B-chains in preparations of the separate polypeptides purified by DEAE Bio-Gel A chromatography and by chromatofocusing.Entities:
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Year: 1982 PMID： 7150646 DOI： 10.1016/0304-4165(82)90108-8
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002