Literature DB >> 7142712

One-step purification of mouse monoclonal antibodies from ascitic fluid by DEAE Affi-Gel blue chromatography.

C Bruck, D Portetelle, C Glineur, A Bollen.   

Abstract

Monoclonal antibodies can be purified directly from ascitic fluids by chromatography on a DEAE Affi-gel blue column. Optimal conditions were determined for the recovery of immunoglobulins free of contaminating protease and nuclease activities.

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Year:  1982        PMID： 7142712     DOI： 10.1016/0022-1759(82)90178-8

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  60 in total

1.  Preparation and purification of F(ab')(2) fragment from anti hepatoma mouse IgG(1) mAb.

Authors:  Cheng-Gang Liu; Mei-Cai Zhu; Zhi-Nan Chen
Journal:  World J Gastroenterol       Date:  1999-12       Impact factor: 5.742

2.  Analytical approaches to the study of monoclonal antibody stability.

Authors:  W Jiskoot; E C Beuvery; A A de Koning; J N Herron; D J Crommelin
Journal:  Pharm Res       Date:  1990-12       Impact factor: 4.200

3.  S2 episome of maize mitochondria encodes a 130-kilodalton protein found in male sterile and fertile plants.

Authors:  G Zabala; C O'brien-Vedder; V Walbot
Journal:  Proc Natl Acad Sci U S A       Date:  1987-11       Impact factor: 11.205

4.  Production and characterization of monoclonal antibodies to polyomavirus major capsid protein VP1.

Authors:  S J Marriott; R A Consigli
Journal:  J Virol       Date:  1985-11       Impact factor: 5.103

5.  The use of monoclonal antibodies to analyze the structure of Clostridium botulinum type E derivative toxin.

Authors:  S Kozaki; Y Kamata; T Nagai; J Ogasawara; G Sakaguchi
Journal:  Infect Immun       Date:  1986-06       Impact factor: 3.441

6.  Immunochemical characterization of a protein associated with Mycobacterium leprae cell wall.

Authors:  T P Gillis; R A Miller; D B Young; S R Khanolkar; T M Buchanan
Journal:  Infect Immun       Date:  1985-08       Impact factor: 3.441

7.  Production and characterization of monoclonal antibodies against Clostridium perfringens type A enterotoxin.

Authors:  A P Wnek; R J Strouse; B A McClane
Journal:  Infect Immun       Date:  1985-11       Impact factor: 3.441

8.  Serodiagnosis of leprosy: relationships between antibodies to Mycobacterium leprae phenolic glycolipid I and protein antigens.

Authors:  W R Levis; H C Meeker; G B Schuller-Levis; T P Gillis; L J Marino; J Zabriskie
Journal:  J Clin Microbiol       Date:  1986-12       Impact factor: 5.948

9.  Roles of apolipoproteins B and E in the cellular binding of very low density lipoproteins.

Authors:  E S Krul; M J Tikkanen; T G Cole; J M Davie; G Schonfeld
Journal:  J Clin Invest       Date:  1985-02       Impact factor: 14.808

10.  Sensitive and specific two-site immunoradiometric assays for human insulin, proinsulin, 65-66 split and 32-33 split proinsulins.

Authors:  W J Sobey; S F Beer; C A Carrington; P M Clark; B H Frank; I P Gray; S D Luzio; D R Owens; A E Schneider; K Siddle
Journal:  Biochem J       Date:  1989-06-01       Impact factor: 3.857
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