Literature DB >> 7140720

Measurement of uroporphyrinogen decarboxylase using porphyrinogens prepared by chemical reduction.

G H Elder, P C Wyvill.   

Abstract

A sensitive, specific method for the measurement of uroporphyrinogen decarboxylase(porphyrinogen carboxy-lyase, EC4.1.1.37) in liver homogenates, haemolysates and other tissues is described. Pentacarboxylic porphyrinogen III, prepared from the corresponding porphyrin by reduction with sodium-mercury amalgam, is used as substrate. After oxidation and methyl esterification the reaction product, coproporphyrinogen III, is separated from the substrate and measured by high-performance liquid chromatography, with mesoporphyrin IX as internal standard. The method can readily be adapted for use with any of the other substrates of uroporphyrinogen decarboxylase, including uroporphyrinogens I and III.

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Year:  1982        PMID： 7140720     DOI： 10.1159/000459101

Source DB:  PubMed          Journal:  Enzyme        ISSN： 0013-9432


  7 in total

1.  Modified uroporphyrinogen decarboxylase activity in a yeast mutant which mimics porphyria cutanea tarda.

Authors:  J Rytka; T Bilinski; R Labbe-Bois
Journal:  Biochem J       Date:  1984-03-01       Impact factor: 3.857

2.  Inhibition of uroporphyrinogen decarboxylase by halogenated biphenyls in chick hepatocyte cultures. Essential role for induction of cytochrome P-448.

Authors:  P R Sinclair; W J Bement; H L Bonkovsky; J F Sinclair
Journal:  Biochem J       Date:  1984-09-15       Impact factor: 3.857

3.  Uroporphyria produced in mice by 20-methylcholanthrene and 5-aminolaevulinic acid.

Authors:  A J Urquhart; G H Elder; A G Roberts; R W Lambrecht; P R Sinclair; W J Bement; N Gorman; J A Sinclair
Journal:  Biochem J       Date:  1988-07-15       Impact factor: 3.857

4.  Hepatic uroporphyrin accumulation and uroporphyrinogen decarboxylase activity in cultured chick-embryo hepatocytes and in Japanese quail (Coturnix coturnix japonica) and mice treated with polyhalogenated aromatic compounds.

Authors:  R W Lambrecht; P R Sinclair; W J Bement; J F Sinclair; H M Carpenter; D R Buhler; A J Urquhart; G H Elder
Journal:  Biochem J       Date:  1988-07-01       Impact factor: 3.857

5.  Purification of uroporphyrinogen decarboxylase from human erythrocytes. Immunochemical evidence for a single protein with decarboxylase activity in human erythrocytes and liver.

Authors:  G H Elder; J A Tovey; D M Sheppard
Journal:  Biochem J       Date:  1983-10-01       Impact factor: 3.857

6.  Iron and the liver: acute effects of iron-loading on hepatic heme synthesis of rats. Role of decreased activity of 5-aminolevulinate dehydrase.

Authors:  H L Bonkowsky; J F Healey; P R Sinclair; J F Sinclair; S I Shedlofsky; G H Elder
Journal:  J Clin Invest       Date:  1983-05       Impact factor: 14.808

7.  Heterogeneity of familial porphyria cutanea tarda.

Authors:  A G Roberts; G H Elder; R G Newcombe; R Enriquez de Salamanca; J J Munoz
Journal:  J Med Genet       Date:  1988-10       Impact factor: 6.318
  7 in total

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