[Effect of irradiation on microviscosity of the cellular nuclear membrane of tumor and liver of tumor-carriers].

Spin probe analysis was carried out of changes in microviscosity of the nuclear membranes of the cells of tumor and liver of tumor-carriers with developing Ehrlich ascite carcinoma (EAC) different time after irradiation with lethal doses (650 r). Two iminoxyl radicals mainly localized in a lipid bilayer and in the membrane lipid layers adjacent to protein were applied. The pattern and level of changes in microviscosity of different nuclear membrane regions point to different response to irradiation of the cells of the tumor-carrier organ and of the tumor, both in viscosity properties and in the changes in lipid-protein relationships. A significant contribution to the total changes of the nuclear membrane microviscosity was made by the lipid layers adjacent to proteins. It is responsible for different reactions of the nuclear membranes of liver cells in tumor-carriers and in healthy animals, and also for a significant (3-fold) dilution of the nuclear membrane of EAC cells after irradiation. It is shown that temperature relationship of the times of rotative correlation of both probes is more pronounced in EAC cells of irradiated tumor-carriers than in the liver.