Literature DB >> 7138858

Kinetic characterization and substrate requirement for the Ca2+ uptake system in platelet membrane.

M A Javors, C L Bowden, D H Ross.   

Abstract

An ATP-dependent mechanism for Ca2+ uptake in human platelet membrane fractions has been identified and characterized. Ca2+ uptake into a membrane fraction is shown to be stimulated at low concentrations of ATP and Ca2+ and to require magnesium ions. Initial rate kinetics, using Eadie-Scatchard analysis, indicated a single class of calcium uptake sites in the presence of ATP, with a Kd for free [Ca2+] of 0.145 microM. Ca2+ uptake in the presence of several ATP concentrations demonstrates that ATP binds to at least two sites, representing high and low affinities of 3.21 and 80.1 microM, respectively. The neuroleptic drug fluphenazine inhibited ATP-stimulated calcium uptake (IC50 = 55 microM), suggesting this ATP-dependent Ca2+ uptake system may provide a useful ion-transport model with which to study neuroleptic therapy in humans.

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Year:  1982        PMID： 7138858     DOI： 10.1016/0005-2736(82)90410-2

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  6 in total

1.  The identification of sarcoplasmic reticulum terminal cisternae proteins in platelets.

Authors:  T H Fischer; D W Barton; K H Krause; T E White; K P Campbell; G C White
Journal:  Biochem J       Date:  1989-10-15       Impact factor: 3.857

2.  Calcium efflux from platelet vesicles of the dense tubular system. Analysis of the possible contribution of the Ca2+ pump.

Authors:  R G Teijeiro; J R Sotelo Silveira; J R Sotelo; J C Benech
Journal:  Mol Cell Biochem       Date:  1999-09       Impact factor: 3.396

3.  Reversal of the Ca2+ pump of blood platelets.

Authors:  J C Benech; H Wolosker; L de Meis
Journal:  Biochem J       Date:  1995-02-15       Impact factor: 3.857

4.  The phosphoprotein that regulates platelet Ca2+ transport is located on the plasma membrane, controls membrane-associated Ca2(+)-ATPase and is not glycoprotein Ib beta-subunit.

Authors:  A Darnanville; R Bredoux; K J Clemetson; N Kieffer; N Bourdeau; S Levy-Toledano; J P Caen; J Enouf
Journal:  Biochem J       Date:  1991-01-15       Impact factor: 3.857

5.  Human platelets express the SERCA2-b isoform of Ca(2+)-transport ATPase.

Authors:  J Enouf; R Bredoux; B Papp; I Djaffar; A M Lompré; N Kieffer; O Gayet; K Clemetson; F Wuytack; J P Rosa
Journal:  Biochem J       Date:  1992-08-15       Impact factor: 3.857

6.  Platelet intracellular calcium in patients with recurrent affective disorders.

Authors:  R A Bothwell; D Eccleston; E Marshall
Journal:  Psychopharmacology (Berl)       Date:  1994-03       Impact factor: 4.530
  6 in total

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