beta 2-Tubulin, a form of chordate brain tubulin with lesser reactivity toward an assembly-inhibiting sulfhydryl-directed cross-linking reagent.

Beta 1 and beta 2 are the designations given to two forms of beta-tubulin that have different electrophoretic mobilities on discontinuous polyacrylamide gels in the presence of sodium dodecyl sulfate [Little, M. (1979) FEBS Lett. 108, 283-286]. Beta 1 and beta 2 constitute respectively 75% and 25% of the total beta-tubulin in bovine brain. Although beta 1 appears to be ubiquitous in animals, beta 2 has so far only been found in the brains of cows, pigs, deer, rats, chicks, and dogfish but not in squid brain. Beta 2 is not found in bovine kidneys, in porcine lungs, or in any nonchordate tubulin that has been examined. When tubulin is reacted with the sulfhydryl-directed reagent N,-N'-ethylenebis(iodoacetamide) (EBI), beta 1, but not beta 2, is converted to a faster moving form, beta. The yield of beta 2 in this reaction is not altered by the presence of drugs. When [14C]EBI is used as a probe, most of the label is incorporated into beta 1 rather than beta 2. Tubulin molecules that have reacted with EBI to form beta are much less likely to polymerize into microtubules than are molecules that have not formed beta. In view of the observation that only beta 1, and not beta 2, can form beta, it is possible that beta 1 represents a form of tubulin whose assembly may be regulated by a mechanism involving sulfhydryls. In contrast, beta 2 may represent a form of tubulin whose assembly is regulated by some other mechanism.