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Literature DB >> 7134000

Blotting of RNA onto ion exchange paper allowing subsequent characterization by in situ translation in addition to blot hybridization.

C J Saris, H J Franssen, J H Heuyerjans, J van Eenbergen, H P Bloemers.

Abstract

We present the preparation of an ion exchange paper which can be used as a solid carrier in the transfer of RNA from gels. In addition to detection by blot hybridization to specific probes, transferred RNA can be characterized by cell-free translation in situ. Preparation of the paper is simple, inexpensive and reproducible. Examples of applications are shown and possible other applications are discussed.

Mesh：

Substances：
RNA, Messenger
RNA, Viral

Year: 1982 PMID： 7134000 PMCID： PMC320821 DOI： 10.1093/nar/10.16.4831

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

12 in total

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

2. Translation of MuLV and MSV RNAs in nuclease-treated reticulocyte extracts: enhancement of the gag-pol polypeptide with yeast suppressor tRNA.

Authors: L Philipson; P Andersson; U Olshevsky; R Weinberg; D Baltimore; R Gesteland
Journal: Cell Date: 1978-01 Impact factor: 41.582

3. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.

Authors: J C Alwine; D J Kemp; G R Stark
Journal: Proc Natl Acad Sci U S A Date: 1977-12 Impact factor: 11.205

4. Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid.

Authors: R D Palmiter
Journal: Biochemistry Date: 1974-08-13 Impact factor: 3.162

5. The synthesis of celluloses containing covalently bound nucleotides, polynucleotides, and nucleic acids.

Authors: P T Gilham
Journal: Biochemistry Date: 1968-08 Impact factor: 3.162

6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

7. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

Authors: P S Thomas
Journal: Proc Natl Acad Sci U S A Date: 1980-09 Impact factor: 11.205

8. Preparation and preliminary characterization of purified ovalbumin messenger RNA from the hen oviduct.

Authors: J M Rosen; S L Woo; J W Holder; A R Means; B W O'Malley
Journal: Biochemistry Date: 1975-01-14 Impact factor: 3.162

9. An efficient mRNA-dependent translation system from reticulocyte lysates.

Authors: H R Pelham; R J Jackson
Journal: Eur J Biochem Date: 1976-08-01

10. An improved method for the preparation of undegraded polysomes and active messenger RNA from immature chicken erythrocytes.

Authors: M Perucho; H V Molgaard; A Shevack; T Pataryas; A Ruiz-Carrillo
Journal: Anal Biochem Date: 1979-10-01 Impact factor: 3.365

5 in total

5. Neuroblastoma cells express c-sis and produce a transforming growth factor antigenically related to the platelet-derived growth factor.

Authors: E J van Zoelen; W J van de Ven; H J Franssen; T M van Oostwaard; P T van der Saag; C H Heldin; S W de Laat
Journal: Mol Cell Biol Date: 1985-09 Impact factor: 4.272