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Literature DB >> 7130272

The Z-band: 85,000-dalton amorphin and alpha-actinin and their relation to structure.

Abstract

The conclusions arrived at as a result of this work can be summarized as follows: (a) We have found that there is an 85,000-dalton protein, which we have called 85K amorphin, associated with the Z-band of chicken pectoralis muscle myofibrils. We have isolated and purified this protein. It is not a structural component of the Z-filaments since it can be extracted completely without extraction of the Z-filaments. Extraction of 85K amorphin results in loss of specific staining of the Z-band with fluorescence specific anti-85K amorphin. (b) We have found that alpha-actinin is the structural component of the Z-filaments, since extraction of alpha-actinin is accompanied by loss of the Z-filament structure.

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Year: 1982 PMID： 7130272 PMCID： PMC2112221 DOI： 10.1083/jcb.94.3.565

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

34 in total

The Z-band: 85,000-dalton amorphin and alpha-actinin and their relation to structure.

1. The myosin filament. I. Structural organization from antibody staining observed in electron microscopy.

2. The modification of actomyosin by alpha-actinin. 3. The enteraction between alpha-actinin and actin.

3. An improved method for the preparation of alpha-actinin from rabbit striated muscle.

4. Purification and properties of alpha-actinin from rabbit skeletal muscle.

5. [Study of a new observation of "nemaline myopathy". II. Ultrastructural findings].

6. Localization of 6S component of a alpha-actinin at Z-band.

7. The influence of fixation upon the fine structure of the Z-disk of rat striated muscle.

8. The effect of various protein fractions on Z- and M-line reconstitution.

9. Models of muscle Z-band fine structure based on a looping filament configuration.

10. Some aspects of the structural organization of the myofibril as revealed by antibody--staining methods.

1. Vertebrate muscle Z-line structure: an electron microscopic study of negatively-stained myofibrils.

2. Identification of lipids as the main component of skeletal muscle Z-discs.

3. DNase I interactions with filaments of skeletal muscles.

4. Immunocytochemical studies using a monoclonal antibody to bovine cardiac titin on intact and extracted myofibrils.

Review 5. The muscle Z band: lessons in stress management.

6. Novel staining pattern of skeletal muscle M-lines upon incubation with antibodies against MM-creatine kinase.

7. Tropomodulin is associated with the free (pointed) ends of the thin filaments in rat skeletal muscle.

8. Three-dimensional reconstruction of a simple Z-band in fish muscle.

9. Characterization of components of Z-bands in the fibrillar flight muscle of Drosophila melanogaster.

10. Formation and alignment of Z lines in living chick myotubes microinjected with rhodamine-labeled alpha-actinin.